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A portable intelligent hydrogel platform for multicolor visual detection of HAase

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Abstract

Hyaluronidase (HAase) is an important endoglycosidase involved in numerous physiological and pathological processes, such as apoptosis, senescence, and cancer progression. Simple, convenient, and sensitive detection of HAase is important for clinical diagnosis. Herein, an easy-to-operate multicolor visual sensing strategy was developed for HAase determination. The proposed sensor was composed of an enzyme-responsive hydrogel and a nanochromogenic system (gold nanobipyramids (AuNBPs)). The enzyme-responsive hydrogel, formed by polyethyleneimine-hyaluronic acid (PEI-HA), was specifically hydrolyzed with HAase, leading to the release of platinum nanoparticles (PtNPs). Subsequently, PtNPs catalyzed the mixed system of 3,3′,5,5′-tetramethylbenzidine (TMB) and H2O2 to produce TMB2+ under acidic conditions. Then, TMB2+ effectively etched the AuNBPs and resulted in morphological changes in the AuNBPs, accompanied by a blueshift in the localized surface plasmon resonance peak and vibrant colors. Therefore, HAase can be semiquantitatively determined by directly observing the color change of AuNBPs with the naked eye. On the basis of this, the method has a linear detection range of HAase concentrations between 0.6 and 40 U/mL, with a detection limit of 0.3 U/mL. In addition, our designed multicolor biosensor successfully detected the concentration of HAase in human serum samples. The results showed no obvious difference between this method and enzyme-linked immunosorbent assay, indicating the good accuracy and usability of the suggested method.

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Funding

This project was supported by the National Natural Science Foundation of China (no. 22264018); Jiangxi Provincial Natural Science Foundation (20224BAB213005, 20232BAB216118, 20232BAB203019); the Science and technology research project of Jiangxi Provincial Department of Education (GJJ201253); and the Doctoral Scientific Research Foundation of Jiangxi University of Chinese Medicine (2022BSZR008, 2021BSZR013, 2020BSZR006).

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Author notes

  1. Zhe Zhao and Zhixin Li contributed equally to this work.

Authors and Affiliations

  1. Cancer Research Center& Jiangxi Engineering Research Center for Translational Cancer Technology, Jiangxi University of Chinese Medicine, Nanchang, 330004, Jiangxi, China

    Zhe Zhao, Jiahui Huang, Ray P.S. Han, Yingzhou Tao & Shaohua Xu

  2. Institute for Advanced Study, Research Center for Differentiation and Development of TCM Basic Theory, Jiangxi University of Chinese Medicine, Nanchang, 330004, Jiangxi, China

    Zhixin Li

  3. Ministry of Education Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Jiangxi University of Chinese Medicine, Nanchang, 330004, Jiangxi, China

    **aoyu Deng

  4. School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang, 330004, Jiangxi, China

    Fan Jiang

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  1. Zhe Zhao
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Contributions

Zhe Zhao: carried out experiments, wrote the manuscript. Zhixin Li: carried out experiments, corrected, and edited. Jiahui Huang: manuscript reviewed, corrected, and edited. **aoyu Deng: technique support, secured funding. Fang Jiang: revised the manuscript. Ray P. S. Han: theoretical support. Yingzhou Tao: instructed the students, revised the manuscript and secured funding. Shaohua Xu: designed the experiments, instructed the students, and secured funding.

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Correspondence to Ray P.S. Han, Yingzhou Tao or Shaohua Xu.

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Zhao, Z., Li, Z., Huang, J. et al. A portable intelligent hydrogel platform for multicolor visual detection of HAase. Microchim Acta 191, 101 (2024). https://doi.org/10.1007/s00604-024-06181-y

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