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FeNC nanozyme-based electrochemical immunoassay for sensitive detection of human epidermal growth factor receptor 2

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Abstract

The proof-of-concept of sensitive electrochemical immunoassay for the quantitative monitoring of human epidermal growth factor receptor 2 (HER2) is reported. The assay is carried out on iron nitrogen-doped carbon (FeNC) nanozyme-modified screen-printed carbon electrode using chronoamperometry. Introduction of target HER2 can induce the sandwiched immunoreaction between anti-HER2 monoclonal antibody-coated microplate and biotinylated anti-HER2 polyclonal antibody. Thereafter, streptavidin-glucose oxidase (GOx) conjugate is bonded to the detection antibody. Upon addition of glucose, 3,3′,5,5′-tetramethylbenzidine (TMB) is oxidized through the produced H2O2 with the assistance of GOx and FeNC nanozyme. The oxidized TMB is determined via chronoamperometry. Experimental results revealed that electrochemical immunosensing system exhibited good amperometric response, and allowed the detection of target HER2 as low as 4.5 pg/mL. High specificity and long-term stability are acquired with FeNC nanozyme-based sensing strategy. Importantly, our system provides a new opportunity for protein diagnostics.

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Funding

Authors acknowledged financial support from the National Natural Science Foundation of China (Grant nos.: 22274022 and 21874022).

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Authors and Affiliations

  1. Key Laboratory of Analytical Science for Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou, 350108, People’s Republic of China

    Minghao Qiu, Yuqing Ren, Lumin Huang, Xueying Zhu, Tikai Liang, Mei** Li & Dian** Tang

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  1. Minghao Qiu
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  2. Yuqing Ren
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  3. Lumin Huang
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  4. Xueying Zhu
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  5. Tikai Liang
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  6. Mei** Li
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  7. Dian** Tang
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Contributions

Mingha Qiu: conceptualization, experimentation, writing—original draft, formal analysis, and investigation. Yuqing Ren: experimentation, formal analysis, and investigation. Lumin Huang: experimentation and formal analysis. Xueying Zhu: experimentation and formal analysis. Tikai Liang: experimentation and formal analysis. Mei** Li: material characterization and data analysis. Dian** Tang: conceptualization, supervision, funding acquisition, resource, and writing—review and editing.

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Correspondence to Mei** Li or Dian** Tang.

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All experiments were performed in compliance with the relevant laws and Guidelines of Fuzhou University (China), and the experiments have been approved.

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Qiu, M., Ren, Y., Huang, L. et al. FeNC nanozyme-based electrochemical immunoassay for sensitive detection of human epidermal growth factor receptor 2. Microchim Acta 190, 378 (2023). https://doi.org/10.1007/s00604-023-05964-z

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