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Electrochemical label-free immunoassay of HE4 using 3D PtNi nanocubes assemblies as biosensing interfaces

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Abstract

Human epididymis protein 4 (HE4) is a vital biomarker for early diagnosis of epithelial ovarian cancer (EOC). Herein, a new label-free biosensor was developed using K3[Fe(CN)6] as the electrochemical probe for ultrasensitive immunoassay of HE4 based on PtNi nanocubes assemblies (NCAs) as efficient biosensing interfaces. The PtNi NCAs were synthesized by a simple solvothermal approach, where N-hexadecyltrimethylammonium chloride (HTAC) and 2,2′-bis(4,5-dimethylimidazole) (BDMM) behaved as co-structuring directors. Under the optimal conditions, the obtained HE4 immunosensor displayed a wide detection range from 0.001 to 100 ng mL−1 and a low detection limit (0.11 pg mL−1, S/N = 3). As a result, the current sensing platform would serve as a useful reference for detecting cancer biomarkers in the clinical assay and diagnosis.

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Funding

This research was supported by Zhejiang Public Welfare Technology Application Research Project (LGG19B050001) and National Students’ Innovation and Entrepreneurship Training Program of Zhejiang Normal University (202210345026).

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Author notes

  1. Di-Nan Chen and Lu-Yao Jiang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Life Sciences, College of Geography and Environmental Sciences, Zhejiang Normal University, **hua, 321004, China

    Di-Nan Chen, Lu-Yao Jiang, **g-**an Zhang, Chang Tang, Ai-Jun Wang & Jiu-Ju Feng

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  1. Di-Nan Chen
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  2. Lu-Yao Jiang
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  3. **g-**an Zhang
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  4. Chang Tang
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Correspondence to Jiu-Ju Feng.

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Research highlights.

•PtNi nanocube assemblies were synthesized by a solvothermal method.

•BDMM and HTAC were acted as co-structuring directors.

•PtNi NCAs showed highly catalytic activity.

•The hierarchical superstructure provided abundant binding sites.

•The immunosensor was applied for ultrasensitive detection of HE4.

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Supplementary file1 (DOCX 542 KB)

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Chen, DN., Jiang, LY., Zhang, JX. et al. Electrochemical label-free immunoassay of HE4 using 3D PtNi nanocubes assemblies as biosensing interfaces. Microchim Acta 189, 455 (2022). https://doi.org/10.1007/s00604-022-05553-6

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