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Stability improvement of polyaniline nanocomposite immunosensor for early detection of insulin receptor antibody as biomarker of type 2 diabetes

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Abstract

Polyaniline nanocomposite with controllable properties was used to design and fabricate a novel electrochemical immunosensor for the early detection of type 2 diabetes. Insulin receptor antibody is a powerful predictor of type 2 diabetes development in individuals. A systematic study was carried out to investigate the effects of different polyaniline layers and the Nafion layer on the morphological, chemical, and electrochemical properties of nanocomposite immunosensor, especially the stability. The bioengineered Nafion-Au nanoparticles-polyaniline/gold electrode demonstrated outstanding electrocatalytic performance in the detection of insulin receptor antibodies with a high sensitivity (136.21 µA.ng−1.ml.cm−2) in a linear range from 0.001 to 200 ng.ml−1 as well as a low detection limit of 1.827 pg.ml−1, response time within 10 min, remarkable selectivity, and significant stability of 80 days. Therefore, the developed immunosensor is a suitable nanocomposite platform for insulin receptor antibody level determination in human plasma.

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Funding

This research was financially supported by the Iran National Science Foundation [funding reference number-97025269], the University of Tehran’s Research Center of New Technologies in Life Science Engineering (UTLSE), the University of British Columbia, the Canada Research Chairs program, and the Natural Sciences and Engineering Research Council of Canada (NSERC) [Discovery Grant-RGPIN-2020–03914].

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Authors and Affiliations

  1. School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, 11155-4563, Iran

    Mohammadreza Farrokhnia & Ghassem Amoabediny

  2. Nanomaterials and Polymer Nanocomposites Laboratory, School of Engineering, University of British Columbia, Kelowna, BC, V1V 1V7, Canada

    Mohammadreza Farrokhnia & Mohammad Arjmand

  3. Research Center of New Technologies in Life Science Engineering, University of Tehran, Tehran, 14179-63891, Iran

    Mohammadreza Farrokhnia, Ghassem Amoabediny & Mohammad Ebrahimi

  4. Medical Scientific Center for Development of Bioelectric and Bio-Feedback Technologies, Tehran, Iran

    Mohammad Ebrahimi

  5. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, 14174-66191, Iran

    Mohammad Reza Ganjali

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  1. Mohammadreza Farrokhnia
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  2. Ghassem Amoabediny
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  3. Mohammad Ebrahimi
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  4. Mohammad Reza Ganjali
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Mohammadreza Farrokhnia: research design; formal analysis; data curation; role/writing—original draft; validation; methodology; experimental. Ghassem Amoabediny: conceptualization; formal analysis; project administration; supervision; funding acquisition; writing—review and editing. Mohammad Ebrahimi: advision; conceptualization; writing—review and editing. Mohammadreza Ganjali: writing—review and editing; advision. Mohammad Arjmand: formal analysis; supervision; funding acquisition; writing—review and editing; supplying experimental tests.

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Farrokhnia, M., Amoabediny, G., Ebrahimi, M. et al. Stability improvement of polyaniline nanocomposite immunosensor for early detection of insulin receptor antibody as biomarker of type 2 diabetes. Microchim Acta 189, 439 (2022). https://doi.org/10.1007/s00604-022-05503-2

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