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Voltammetric determination of organophosphorus pesticides using a hairpin aptamer immobilized in a graphene oxide-chitosan composite

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Abstract

An aptasensor is described for electrochemical determination of organophosphorus pesticides (OPPs), specifically of profenofos, phorate, isocarbophos, and omethoate. The method uses a hairpin aptamer as signalling donor. Its 5′ and 3′ ends were modified with amino groups and the redox probe ferrocene (Fc), respectively. A nanocomposite consisting of graphene oxide and chitosan (GO-chit) was used to immobilize the aptamer via formation of an amide link. Its good conductivity facilitates monitoring of the electrochemical responses. Upon addition of an OPP, it will be bound by the aptamer. This results in an opening of the hairpin structure. Thus, Fc is shifted away from the surface of the electrode. As a result, the impedance increases and the redox signal of Fc decreases. The electrochemical performance, binding capacity and response of the aptasensor for profenofos, phorate, isocarbophos and omethoate were studied. The limits of detection are as low as 0.01, 0.1, 0.01 and 0.1 nM, respectively.

Schematic representation of an electrochemical aptasensor prepared by immobilizing ferrocene (Fc) labeled hairpin aptamer (HP) on the surface of graphene oxide-chitosan (GO-chit) modified electrode, and its application to the determination of organophosphorus pesticides (OPPs) by voltammetry.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31772068, 31872909), Special Project of Independent Innovation of Shandong Province (2018CXGC0214), Shandong Provincial Natural Science Foundation (ZR2018ZC0126).

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Authors and Affiliations

  1. School of Agriculture Engineering and Food Science, Shandong University of Technology, No.12 Zhangzhou Road, Zibo, 255049, Shandong Province, People’s Republic of China

    Jiayun Fu, Yao Yao, **ngshuang An, Guangxian Wang, Yemin Guo, **a Sun & Falan Li

  2. Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability, No.12 Zhangzhou Road, Zibo, 255049, Shandong Province, People’s Republic of China

    Yemin Guo, **a Sun & Falan Li

  3. Zibo City Key Laboratory of Agricultural Product Safety Traceability, Shandong, China

    Yemin Guo, **a Sun & Falan Li

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  1. Jiayun Fu
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Correspondence to Yemin Guo or **a Sun.

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Fu, J., Yao, Y., An, X. et al. Voltammetric determination of organophosphorus pesticides using a hairpin aptamer immobilized in a graphene oxide-chitosan composite. Microchim Acta 187, 36 (2020). https://doi.org/10.1007/s00604-019-4022-4

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