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An organic polymer monolith modified with an amino acid ionic liquid and graphene oxide for use in capillary electrochromatography: application to the separation of amino acids, β-blockers, and nucleotides

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Abstract

The preparation of an organic polymer monolithic column modified with an amino acid ionic liquid and graphene oxide (AAIL-GO) and its application to capillary electrochromatography (CEC) was described. The AAIL tetramethylammonium-L-arginine was bonded to a monolithic column that was previously modified with graphene oxide by using an hydrochloride/N-hydroxysuccinimide coupling reaction. The morphology of a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith was examined by scanning electron microscopy. The incorporation of AAIL and graphene oxide was detected by infrared spectroscopy and elemental analysis. The resulting monolithic column produced a strong and stable electroosmotic flow from the anode to the cathode in the pH range from 3 to 9. Compared with a column modified with AAIL or graphene oxide only, the AAIL-GO-modified column has a better separation ability for amino acids, β-blockers, and nucleotides (the resolution of three amino acids: 2.231 and 2.036, β-blockers: 2.779 and 2.470 and nucleotides: 8.345 and 3.321). Molecular modeling was applied to demonstrate the separation mechanism of small molecules which showed a good support for experimental results.

Schematic representation of capillary electrochromatography (CEC) systems with an amino acid ionic liquid-graphene oxide modified organic polymer monolithic column as stationary phases for separation of amino acids, β-blockers, and nucleotides.

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Acknowledgements

This work was supported by the Natural Science Foundation of Jiangsu Province (Program No.: BK20141353).

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Authors and Affiliations

  1. Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, No.24 Tongjiaxiang, Nan**g, Jiangsu, 210009, People’s Republic of China

    Shiyuan Zhao, Tao Yu, Yingxiang Du, **aodong Sun, Zijie Feng, **aofei Ma, Wen Ding & Cheng Chen

  2. State Key Laboratory of Natural Medicines, China Pharmaceutical University, No.24 Tongjiaxiang, Nan**g, Jiangsu, 210009, People’s Republic of China

    Shiyuan Zhao, Tao Yu, Yingxiang Du, **aodong Sun, Zijie Feng, **aofei Ma, Wen Ding & Cheng Chen

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  1. Shiyuan Zhao
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Correspondence to Tao Yu or Yingxiang Du.

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Zhao, S., Yu, T., Du, Y. et al. An organic polymer monolith modified with an amino acid ionic liquid and graphene oxide for use in capillary electrochromatography: application to the separation of amino acids, β-blockers, and nucleotides. Microchim Acta 186, 636 (2019). https://doi.org/10.1007/s00604-019-3723-z

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