Abstract
We report on the application of an automated and easy-to-use device to directly measure the immunoreactions between adda-specific monoclonal antibodies and microcystins. The antibodies were immobilized on a gold electrode whose surface was modified first with polytyramine and then with gold nanoparticles. The immunoreaction leads to a change in the capacitance of the system. Under optimum conditions, the sensor is capable of performing stable regeneration-assay cycles and has a low detection limit at a concentration of 0.01 pM level of microcystin-leucine-arginine (MC-LR). The surface of the biosensor can be regenerated with pH 2.5 glycine buffer which dissociates the antibody-antigen complex. The biosensor was used to monitor the production of microcystins during batch cultivation of Microcystis aeruginosa (isolated from ponds in Botswana). Liquid chromatography coupled to MS/MS detection was used to identify three variants, viz. MC-LR (995.6 Da), DmMC-LR (981.2 Da) and MC-LA (910.5 Da).
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A capacitive immunosensor was fabricated by immobilizing monoclonal antibodies on a polytyramine-gold nanoparticle layer. The immunosensor was used to quantify microcystins produced by Microcystis aeruginosa; MC-LR, DmMC-LR and MC-LA, and further identified by LC- MS/MS. The results show that cumulative determination of microcystin variants is possible with this immunosensor.
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Botswana International University of Science and Technology is gratefully acknowledged for the financial support. The Research Council of Sweden (VR) also supported a part of this project.
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Lebogang, L., Mattiasson, B. & Hedström, M. Capacitive sensing of microcystin variants of Microcystis aeruginosa using a gold immunoelectrode modified with antibodies, gold nanoparticles and polytyramine. Microchim Acta 181, 1009–1017 (2014). https://doi.org/10.1007/s00604-014-1199-4
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DOI: https://doi.org/10.1007/s00604-014-1199-4