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Fenugreek (Trigonella foenum graecum L.) supplementation safeguards male mice from aflatoxin B1-induced liver and kidney damage

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Abstract

Consumption of plant-derived foods contaminated with aflatoxin and their metabolites in animal products or exposure to contaminated mycotoxins air and dust caused kidney and liver damage. The purpose of this study was to evaluate the effect of fenugreek (Trigonella foenum graecum L.) upon aflatoxin B1-induced damages to the liver and kidney of male albino mice. Thirty mice were divided into five groups, each with six mice. The control group received water and the standard basal diet. The fenugreek group received 200 mg/kg/day of fenugreek orally through an oral gavage tube dissolved in tap water for 4 weeks with a normal basal diet. Aflatoxin group received a diet with aflatoxin B1 at a dose of 20 µg/kg b.wt for 4 weeks. The protective group received fenugreek for 2 weeks and then received dietary aflatoxin for other 2 weeks with the same doses and treatment. The therapeutic group received dietary aflatoxin for 2 weeks with the same doses and treatment of aflatoxin group and then received fenugreek for 2 weeks. Aflatoxin caused liver and kidney damage in addition to the alteration of some hematological parameters that was manifested by increased oxidative damage and a substantial reduction in antioxidant capacity. Decreased antioxidant levels were observed in mice treated with aflatoxin. When compared to normal control mice, there was a significant improvement in liver function, kidney function, and lipid profile. Moreover, aflatoxin significantly increased liver and kidney histopathological and ultrastructural damage. The protective and therapeutic groups significantly improved the hepatotoxicity and nephrotoxicity induced by aflatoxin feeding. Fenugreek seed supplementation was recommended as a regular nutrient for liver and kidney protection against aflatoxin B1 toxicity.

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  1. Faculty of Science, Zoology Department, Suez Canal University, Ismailia, 41522, Egypt

    Heba Nageh Gad EL Hak, Shimaa Ibrahim Metawea & Zohour Ibrahim Nabil

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EL Hak, H.N.G., Metawea, S.I. & Nabil, Z.I. Fenugreek (Trigonella foenum graecum L.) supplementation safeguards male mice from aflatoxin B1-induced liver and kidney damage. Comp Clin Pathol 31, 925–942 (2022). https://doi.org/10.1007/s00580-022-03413-6

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  • DOI: https://doi.org/10.1007/s00580-022-03413-6

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