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Immobilization of urease using Amberlite MB-1

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Abstract

Amberlite MB-1 was used to immobilize urease (EC.3.5.1.5). The thermal stability of the immobilized urease was better than that of the free urease. Its highest activity was obtained at 75 °C and at pH 6.5 while the optimum temperature for the free urease was found to be 25 °C. Urease immobilized on Amberlite MB-1 retained 65% of the original activity after 5 repeated uses and 62% of the activity after 60 days when stored at 4 °C.

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Authors and Affiliations

  1. Institute of Advanced Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia, , , , , , MY

    A. Anita, C. A. Sastry & M. A. Hashim

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  1. A. Anita
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  2. C. A. Sastry
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  3. M. A. Hashim
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Received: 9 December 1996

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Anita, A., Sastry, C. & Hashim, M. Immobilization of urease using Amberlite MB-1. Bioprocess Engineering 17, 355–359 (1997). https://doi.org/10.1007/s004490050397

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  • DOI: https://doi.org/10.1007/s004490050397

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