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Biomethane production and microbial strategies corresponding to high organic loading treatment for molasses wastewater in an upflow anaerobic filter reactor

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Abstract

Molasses wastewater contains high levels of organic compounds, cations, and anions, causing operational problems for anaerobic biological treatment. In this study, an upflow anaerobic filter (UAF) reactor was employed to establish a high organic loading treatment system for molasses wastewater and further investigated the microbial community dynamics in response to this stressful operation. The biogas production increased with an increase in total organic carbon (TOC) loading rate from 1.0 to 14 g/L/day, and then it decreased with further TOC loading rate addition until 16 g/L/day. The UAF reactor achieved a maximum biogas production of 6800 mL/L/day with a TOC removal efficiency of 66.5% at a TOC loading rate of 14 g/L/day. Further microbial analyses revealed that both the bacterial and archaeal communities developed multiple strategies to maintain stable operation of the reactor at high organic loading (e.g., Proteiniphilum and Defluviitoga maintained high abundances throughout the operation; Tissierella temporarily dominated the bacterial community at TOC loading rates of 8.0 to 14 g/L/day; and multi-trophic Methanosarcina shifted as the dominant methanogen at the TOC loading rates of 8.0 to 16 g/L/day). This study presents insights into a high organic loading molasses wastewater treatment system and the microbial flexibility in methane fermentation in response to process disturbances.

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Acknowledgements

This work was supported by the Key Program for International S&T Cooperation Projects of China (2022YFE0108500), and Sichuan Science and Technology Program (23NSFSC1201).

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Authors and Affiliations

  1. College of Architecture and Environment, Sichuan University, Chengdu, 610065, Sichuan, China

    Zhaoyong Sun, **ting He, Yating Chen, Yueqin Tang & Kenji Kida

  2. Institute for Disaster Management and Reconstruction, Sichuan University–Hong Kong Polytechnic University, Chengdu, 610207, China

    Yuwei Chen & Yating Chen

  3. School of Environmental and Planning, Liaocheng University, Liaocheng, 252000, China

    Na Yu

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Sun, Z., He, J., Yu, N. et al. Biomethane production and microbial strategies corresponding to high organic loading treatment for molasses wastewater in an upflow anaerobic filter reactor. Bioprocess Biosyst Eng 46, 1033–1043 (2023). https://doi.org/10.1007/s00449-023-02882-5

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