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Evaluation of enhancing effect of soybean oil on polymalic acid production by Aureobasidium pullulans HA-4D

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Abstract

Polymalic acid (PMA) is a water-soluble polyester produced by Aureobasidium pullulans. In this study, the physiological response of A. pullulans after the addition of vegetable oils was investigated. Soybean oil (SBO) is pivotal for shortening fermentation time and achieving high PMA titer. With the addition of 1% (w/v) SBO, the titer and productivity of PMA was, respectively, increased by 34.2% and 80%. SBO acted as a chemical stimulatory agent rather than a carbon source, the enhancement on PMA production was attributed to the component of fatty acid. SBO induced the dimorphism (yeast-like cells and mycelia) of A. pullulans, in vitro enzyme activities indicated that the TCA oxidative branch for malic acid synthesis might be strengthened, which could generate more ATP for PMA synthesis, and the assay of intracellular energy supply validated this deduction. This study provided a new sight for recognizing the regulatory behavior of SBO in A. pullulans.

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Abbreviations

PMA:

Polymalic acid

SBO:

Soybean oil

TCA:

Tricarboxylic acid

PYC:

Pyruvate carboxylase

ICL:

Isocitrate lyase

MLS:

Malate synthase

IDH:

Isocitrate dehydrogenase

FUM:

Fumarase

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Acknowledgements

This work was supported by the Natural Science Foundation of China (22078122, 22008083), North of Jiangsu Science & Technology Specific Projects (SZ-HA2019005), Major Program of National Natural Science Foundation of Jiangsu Province (19KJA150010), and Major Basic Research Project of the Natural Science Foundation of the Jiangsu Higher Education Institutions (20KJA416004)

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, College of Chemistry and Chemical Engineering, Huaiyin Normal University, Huai’an, 223300, China

    Jun **a, Sili Liu, Jiali Jiao, Zhongyang Qiu, **aoyang Liu, Aiyong He, Ning Xu & Jiaxing Xu

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  1. Jun **a
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  2. Sili Liu
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  7. Ning Xu
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Contributions

JX: investigation, methodology, writing-original draft, and editing; SL: investigation and data curation; JJ: visualization and software; ZQ: validation and funding acquisition; XL: investigation and funding acquisition; AH: resources; NX: methodology; JX: conceptualization, supervision, review & editing, and funding acquisition.

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Correspondence to Jiaxing Xu.

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**a, J., Liu, S., Jiao, J. et al. Evaluation of enhancing effect of soybean oil on polymalic acid production by Aureobasidium pullulans HA-4D. Bioprocess Biosyst Eng 45, 1673–1682 (2022). https://doi.org/10.1007/s00449-022-02772-2

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