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Enhanced nitrogen removal of aerobic denitrifier using extracellular algal organic matter as carbon source: application to actual reservoir water

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Abstract

Man-made organic matter acting as carbon source for oligotrophic aerobic denitrification has been studied extensively, while less attention has been paid to the actual organic matter derived from drinking water reservoir. In this study, the effect of extracellular organic matter (EOM) released from Microcystis aeruginosa and Chlorella sp. and organic matter in actual reservoir water on aerobic denitrification performance of Acinetobacter johnsonii strain WGX-9 has been investigated, by measuring nitrogen removal and determining changes in the properties of organic matter. Results indicated that the Acinetobacter johnsonii strain WGX-9 showed effective nitrogen removal efficiency when cultural conditions were low C/N of 5, pH of 5–11, and low temperature of 8 °C. The nitrate removal efficiency with EOM as the sole carbon source was relatively higher than that with intracellular organic matter or natural organic matter as the sole carbon source. This is probably due to that EOM exerts a lower molecular weight and better ability of donating electrons. Besides, the findings can elucidate that nitrate and total organic matter removal efficiency with actual high-density algal water as the carbon source was higher than that with actual low-density algal water as the carbon source. This was attributed to that more EOM was released in high-density algal water, which highlighted the aerobic denitrification performance of Acinetobacter johnsonii strain WGX-9. This study will provide a reference for the application of aerobic denitrifier in drinking water reservoirs.

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Acknowledgements

This work was supported by the Natural Science Foundation of China (no. 51678472, 51978557), Shaanxi Science Fund for Distinguished Young Scholars (no. 2018JC-026), National Key Research and Development Program of Shaanxi Province (2020ZDLSF06-05), and The Youth Innovation Team of Shaanxi Universities.

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Authors and Affiliations

  1. Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, **’an University of Architecture and Technology, **’an, 710055, People’s Republic of China

    Gang Wen, Tong Wang, Qiqi Wan, Ruihua Cao, Kai Li, **gyi Wang & Tinglin Huang

  2. Shaanxi Key Laboratory of Environmental Engineering, **’an University of Architecture and Technology, **’an, 710055, People’s Republic of China

    Gang Wen, Tong Wang, Qiqi Wan, Ruihua Cao, Kai Li, **gyi Wang & Tinglin Huang

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  1. Gang Wen
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Wen, G., Wang, T., Wan, Q. et al. Enhanced nitrogen removal of aerobic denitrifier using extracellular algal organic matter as carbon source: application to actual reservoir water. Bioprocess Biosyst Eng 43, 1859–1868 (2020). https://doi.org/10.1007/s00449-020-02376-8

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