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Exploring the TLR and NLR signaling pathway relevant molecules induced by the Theileria annulata infection in calves

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Abstract

Theileria annulata is the pathogen of bovine tropical theileriosis. It is extremely harmful to the cattle industry, with huge economic losses. The toll-like receptor (TLR) and NOD-like receptor (NLR) signaling pathways are crucial for resistance to infection of the protozoa, such as Plasmodium falciparum, Toxoplasma gondii, and Trypanosoma cruzi. However, the role of these immune-related pathways is unclear during T. annulata infection. In the present study, peripheral blood mononuclear cells and serum were separated from blood samples of calves infected with homogenized tick supernatants carrying T. annulata sporozoites at 12 h, 24 h, 36 h, 48 h, 72 h, 96 h, 120 h, 144 h and 168 h postinoculation. The Custom RT2 Profiler PCR Array was used to explore the mRNA levels of 42 TLR and NLR signaling pathway relevant genes. The TLR1, TLR6, TLR10, NLRP1, and MyD88 genes and their downstream signaling molecules significantly differed after the T. annulata infection in comparison with that of preinfection from 72 h to 168 h postinoculation. The serum concentrations of IL-6, IL-1β, and TNFα were significantly increased at 96 h and 168 h postinfection. These findings provided novel information to help determine the mechanisms of TLR and NLR signaling pathway involvement in protection against T. annulata infection.

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Financial support

This study was supported by the National Key Basic Research Program (973 program) of China (No. 2015CB150300); the National Key Research and Development Program of China (No. 2017YFD0501200); ASTIP, FRIP (No. 2014ZL010), CAAS; NBCIS CARS-38; NSFC (No. 31372432, 31,402,189); and Jiangsu Co-innovation Center Programme for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, State Key Laboratory of Veterinary Etiological Biology Project. The research was also facilitated by CRP No. 16198/R0 IAEA.

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  1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujia** 1, Lanzhou, 730046, Gansu, People’s Republic of China

    Fangyuan Yin, Junlong Liu, Shandian Gao, Aihong Liu, Shuaiyang Zhao, Sitong Li, **ming Wang, Youquan Li, Jianxun Luo, Guiquan Guan & Hong Yin

  2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, People’s Republic of China

    Hong Yin

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  1. Fangyuan Yin
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  2. Junlong Liu
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  4. Aihong Liu
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  5. Shuaiyang Zhao
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Correspondence to Guiquan Guan or Hong Yin.

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Section Editor: Dana Mordue

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Additional file 1

Detection of T. annulata infection in ticks using the PCR assay. (A) Infected ticks. (B) Uninfected ticks. M: DL2000™ DNA marker. (PNG 64 kb)

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Yin, F., Liu, J., Gao, S. et al. Exploring the TLR and NLR signaling pathway relevant molecules induced by the Theileria annulata infection in calves. Parasitol Res 117, 3269–3276 (2018). https://doi.org/10.1007/s00436-018-6026-0

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