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Cloning and functional characterization of PpDBF1 gene encoding a DRE-binding transcription factor from Physcomitrella patens

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Abstract

The dehydration-responsive element binding (DREB) transcription factors play central roles in regulating expression of stress-inducible genes under abiotic stresses. In the present work, PpDBF1 (P hyscomitrella p atens DRE-binding Factor1) containing a conserved AP2/ERF domain was isolated from the moss P. patens. Sequence comparison and phylogenetic analysis revealed that PpDBF1 belongs to the A-5 group of DREB transcription factor subfamily. The transcriptional activation activity and DNA-binding specificity of PpDBF1 were verified by yeast one-hybrid and electrophoretic mobility shift assay experiments, and its nuclear localization was demonstrated by particle biolisitics. PpDBF1 transcripts were accumulated under various abiotic stresses and phytohormones treatments in P. patens, and transgenic tobacco plants over-expressing PpDBF1 gained higher tolerance to salt, drought and cold stresses. These results suggest that PpDBF1 may play a role in P. patens as a DREB transcription factor, implying that similar regulating systems are conserved in moss and higher plants.

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Acknowledgments

This work was financially supported by the Natural Sciences Foundation of Bei**g (No: KZ200610028017) and the Oriented Projects of the Chinese Academy of Sciences (KSCX2-YW-N-012). We thank Prof. Shou-Yi Chen for technical supports in yeast one-hybrid analysis, and Ms. Hao-Miao Ouyang for help in fluorescence differential display experiment.

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Authors and Affiliations

  1. National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Bei**g, 100101, China

    Ning Liu, Nai-Qin Zhong, Gui-Ling Wang & Gui-**an **a

  2. National Center for Plant Gene Research, Bei**g, 100101, China

    Ning Liu, Nai-Qin Zhong, Gui-Ling Wang & Gui-**an **a

  3. College of Life Science, Capital Normal University, Bei**g, 100037, China

    Li-Juan Li, **ang-Lin Liu & Yi-Kun He

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  1. Ning Liu
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  2. Nai-Qin Zhong
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  3. Gui-Ling Wang
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  4. Li-Juan Li
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Correspondence to Yi-Kun He or Gui-**an **a.

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Liu, N., Zhong, NQ., Wang, GL. et al. Cloning and functional characterization of PpDBF1 gene encoding a DRE-binding transcription factor from Physcomitrella patens . Planta 226, 827–838 (2007). https://doi.org/10.1007/s00425-007-0529-8

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  • DOI: https://doi.org/10.1007/s00425-007-0529-8

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