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Effects of streptozotocin and unilateral nephrectomy on L-type Ca2+ channels and membrane capacitance in arteriolar smooth muscle cells

  • Ion channels, receptors and transporters
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Abstract

Diabetes mellitus and hypertension are common diseases frequently coexisting. Although augmentation of L-type Ca2+ channel (ICaL) activity has been reported in vascular smooth muscle cells (VSMCs) of a spontaneously hypertensive rat model, no study on ICaL has been conducted for coexisting hypertension and diabetes. Sprague Dawley rats were assigned to four groups: a sham-operated control group (CG), a unilateral nephrectomy group (UNG), a streptozotocin (STZ)-induced type 1 diabetic group (SDG) and a coexisting hypertension and diabetes group (DHG), which underwent nephrectomy and received STZ injection. Blood pressure (BP) was significantly lower in the CG than in the other three groups. The membrane capacitance of VSMCs was nearly doubled in the SDG and DHG but not in the UNG. The ICaL was increased approximately 2-fold in both the UNG and SDG and approximately 4-fold in the DHG. The current density of ICaL was increased approximately 2-fold in the UNG and DHG, while no significant increase was seen in the SDG. The rate of Ca2+ removal was inhibited significantly, by ~33 %, in the DHG. In conclusion, the effects of hypertension and diabetes on ICaL were apparently additive, and the vascular consequences of combined diabetes and hypertension may be caused by an elevated ICaL and slowed Ca2+ removal.

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Acknowledgments

KS Park and JB Youm contributed equally to this study. PKS researched the data, YJB wrote the manuscript, JYJ contributed to the discussion and LCH reviewed/edited the manuscript. Prof. Chun Sik Park and Hyun Sook Kim helped generate the hypertension and diabetes animal model. This work was supported by the Bio and Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science, ICT and Future Planning of Korea (No. 20090090901 and No. 2012-0009829).

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Authors and Affiliations

  1. Department of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Inje University, Busan, Korea

    Jae Boum Youm

  2. Department of Physiology, University of Ulsan College of Medicine, 388-1 Poongnap-Dong, Songpa-Gu, Seoul, 138-736, Korea

    Kyung Sun Park, Yeon ** Jang & Chae Hun Leem

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  1. Jae Boum Youm
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Correspondence to Chae Hun Leem.

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Youm, J.B., Park, K.S., Jang, Y.J. et al. Effects of streptozotocin and unilateral nephrectomy on L-type Ca2+ channels and membrane capacitance in arteriolar smooth muscle cells. Pflugers Arch - Eur J Physiol 467, 1689–1697 (2015). https://doi.org/10.1007/s00424-014-1604-1

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  • DOI: https://doi.org/10.1007/s00424-014-1604-1

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