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Proteomics identifies hypothermia induced adiponectin protects corneal endothelial cells via AMPK mediated autophagy in phacoemulsification

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Abstract

Aim

To explore the molecular mechanism underlying the protective effect of hypothermic perfusion on the corneal endothelium during phacoemulsification.

Methods

Phacoemulsification was performed on New Zealand white rabbits. Perfusate at different temperatures was used during the operation, and the aqueous humor was collected for proteomic sequencing after the operation. Corneal endothelial cell injury was simulated by a corneal endothelial cell oxygen–glucose deprivation/reoxygenation (OGD/R) model in vitro. Flow cytometry and evaluation of fluorescent LC3B puncta were used to detect apoptosis and autophagy, and western blotting was used to detect protein expression.

Results

A total of 381 differentially expressed proteins were identified between the two groups. In vitro, 4 ℃ hypothermia significantly reduced apoptosis and promoted autophagy. Apoptosis increased after autophagy was inhibited by 3-Methyladenine (3-MA). Furthermore, adiponectin (ADIPOQ) knockdown inhibited phospho-AMPK and blocked the protective effect of hypothermia on corneal endothelial cells.

Conclusions

We investigated the differential expression of proteins between the hypothermia group and normothermia group by proteomics. Moreover, hypothermia-induced ADIPOQ can reduce apoptosis by promoting AMPK-mediated autophagy.

Key Messages

What is known

• During phacoemulsification, the corneal endothelium may be damaged by ultrasonic energy.

• The use of hypothermic perfusion during phacoemulsification can reduce postoperative inflammation.

What is new

• Hypothermia protects corneal endothelial cells from apoptosis by promoting autophagy.

• Hypothermia induces adiponectin expression and protects corneal endothelial cells through AMPK-mediated autophagy.

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Data availability

The data used to support the results of this study are available from the corresponding authors upon request.

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Funding

This work was supported by the National Natural Science Foundation of China (General Program, Grant No. 81870650, and 81970832), the Project Foundation of Chongqing Science and Technology Commission of China (General Program, Grant No. cstc2021jcyj-msxmX0967, CSTC2021jscx-gksb-N0017 and cstc2022ycjh-bgzxm0121), and the Chongqing Medical University First-class Discipline Construction Project of First Clinical Medical College (PhD Student Research Funding Support Program, No. 472020320220007).

Author information

Authors and Affiliations

  1. The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Lab of Ophthalmology, Chongqing Eye Institute, Chongqing, China

    Yanyi Chen, Kewei Li, Liang **ong, Ruonan Li, Lu Jiang, Yan Xun, Wenjuan Wan & Ke Hu

  2. Department of Endocrinology, Chongqing General Hospital, Chongqing, China

    Rongxi Huang

Authors
  1. Yanyi Chen
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  2. Kewei Li
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  3. Rongxi Huang
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  4. Liang **ong
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  6. Lu Jiang
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  7. Yan Xun
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  8. Wenjuan Wan
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  9. Ke Hu
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Contributions

All the authors contributed to the study conception and design. Yanyi Chen and Kewei Li contributed equally to the work presented here and should therefore be regarded as equivalent first authors; Ke Hu and Wenjuan Wan contributed equally to the work presented here and should therefore be regarded as equivalent corresponding authors. All the authors have read and approved the final manuscript.

Corresponding authors

Correspondence to Wenjuan Wan or Ke Hu.

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Ethical approval

The animal experiments in this research were approved by the Ethics Committee of Chongqing Medical University (2022-K42). This article does not contain any studies with human participants by any of the authors.

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Chen, Y., Li, K., Huang, R. et al. Proteomics identifies hypothermia induced adiponectin protects corneal endothelial cells via AMPK mediated autophagy in phacoemulsification. Graefes Arch Clin Exp Ophthalmol (2024). https://doi.org/10.1007/s00417-024-06542-6

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  • DOI: https://doi.org/10.1007/s00417-024-06542-6

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