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Treatment of femoral head osteonecrosis with advanced cell therapy in sheep

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Abstract

Background

The purpose of this study was to evaluate the efficacy of core decompression associated with advanced cell therapy for the treatment of femoral head osteonecrosis in an established sheep model.

Methods

Early stage osteonecrosis of the right hip was induced cryogenically in 15 mature sheep. At 6 weeks, the sheep were divided into three groups, Group A: core decompression only; Group B: core decompression followed by implantation of an acellular bone matrix scaffold; Group C: core decompression followed by implantation of a cultured BMSC loaded bone matrix scaffold. At 12 weeks, MRI hip studies were performed and then the proximal femur was harvested for histological analysis.

Results

In the group of advanced cell therapy, Group C, there was a tendency to higher values of the relative surface of newly formed bone with a mean of 20.3 versus 11.27 % in Group A and 13.04 % in Group B but it was not statistically significant. However, the mean relative volume of immature osteoid was 8.6 % in Group A, 14.97 in Group B, and 53.49 % in Group C (p < 0.05), revealing a greater capacity of osteoid production in the sheep treated with BMSCs. MRI findings were not conclusive due to constant bone edema artifact in all cases.

Conclusions

Our findings indicate that a BMCSs loaded bone matrix scaffold is capable of stimulating bone regeneration more effectively than isolated core decompression or in association with an acellular scaffold in a preclinical femoral head osteonecrosis model in sheep.

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Acknowledgments

This project received financial support from the Ministerio de Educación, Gobierno de España (Spanish Minsistry of Education, Madrid, Spain) and from Fundació Ferrer Investigació and Fundació Privada A. Bosch (Beguda, Spain). The authors thank Marta Rosal, Marielle Esteves, Alex Rojo and all the personnel at the Institut de Recerca-Hospital Unversitari Vall d’Hebron involved in the project.

Conflict of interest

The authors declare they have no conflict of interest.

Author information

Authors and Affiliations

  1. Orthopaedic Surgery and Traumatology, Hospital Universitario Vall d´Hebron, Pg Vall d’Hebron 129-139, 08035, Barcelona, Spain

    Roberto Vélez, Francisco Soldado, Alejandro Fernández & Marius Aguirre

  2. Orthopaedic Surgery and Traumatology, Hospital Donostia, San Sebastian, Spain

    Alberto Hernández-Fernández

  3. Banc de Sang i Teixits, Divisió de Teràpies Avançades/XCELIA, Barcelona, Spain

    Marta Caminal, Joaquim Vives & Arnau Pla

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  1. Roberto Vélez
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  2. Alberto Hernández-Fernández
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  3. Marta Caminal
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  8. Marius Aguirre
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Correspondence to Roberto Vélez.

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Vélez, R., Hernández-Fernández, A., Caminal, M. et al. Treatment of femoral head osteonecrosis with advanced cell therapy in sheep. Arch Orthop Trauma Surg 132, 1611–1618 (2012). https://doi.org/10.1007/s00402-012-1584-6

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  • DOI: https://doi.org/10.1007/s00402-012-1584-6

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