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Human platelets release TGFBIp in acute myocardial infarction

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Abstract

Transforming growth factor-β-induced protein (TGFBIp) is released from activated platelets and promotes pro-thrombotic complications like pulmonary embolism. The role of TGFBIp in acute coronary syndrome, especially with a focus on platelets, has not been investigated so far. Using ELISA and immunoblotting, we demonstrate platelet TGFBIp release in patients with myocardial infarction (MI). We investigated TGFBIp-induced platelet adhesion and rolling by flow chamber and chemotactic effects of TGFBIp in transwell experiments. Immunochemistry staining of arterial vessels detected TGFBIp and the platelet-specific protein GPVI in the vessel wall.We demonstrate for the first time that platelet TGFBIp release is significantly increased in MI and correlates with the severity of acute coronary syndromes (STEMI, NSTEMI). After activation with TRAP, platelets release TGFBIp and TGFBIp itself activates platelets. Under flow, TGFBIp-mediated platelet rolling and adherence similarly to collagen. TGFBIp significantly increased platelet transmigration and we demonstrate TGFBIp deposits in the wall of human arteries. In this study, we add novel aspects to the role of TGFBIp in acute coronary syndrome by demonstrating that TGFBIp is partially released from platelets during MI and has activating, pro-adhesive and pro-migratory effects on platelets that could contribute to the disease development of coronary vascular inflammation and MI.

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Funding

This work was supported by the Deutsche Forschungsgemeinschaft (DFG:Li849/3–1) to S.L.

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Authors and Affiliations

  1. Medizinische Klinik und Poliklinik I, Klinikum der Universität München, 81377, Munich, Germany

    Bjoern F. Kraemer

  2. Klinik für Herz- und thorakale Gefässchirurgie, Universitätsklinikum Giessen und Marburg, 35043, Marburg, Germany

    Ivo Martinovic

  3. Philipps Universität Marburg, FB 20-Medizin, 35032, Marburg, Germany

    Ivo Martinovic & Stephan Lindemann

  4. Klinikum Warburg, Medizinische Klinik II, Hüffertstr. 50, 34414, Warburg, Germany

    Stephan Lindemann

  5. Medizinische Klinik und Poliklinik III, Universitätsklinikum Tübingen, Tübingen, Germany

    Stephan Lindemann

  6. Faculty of Medicine and Health, JJ Strossmeyer University of Osijek, Osijek, Croatia

    Ivo Martinovic

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  1. Bjoern F. Kraemer
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  2. Ivo Martinovic
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Correspondence to Stephan Lindemann.

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Kraemer, B.F., Martinovic, I. & Lindemann, S. Human platelets release TGFBIp in acute myocardial infarction. Heart Vessels 37, 1962–1970 (2022). https://doi.org/10.1007/s00380-022-02086-z

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  • DOI: https://doi.org/10.1007/s00380-022-02086-z

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