Abstract
A microcosm study was established using three arable soils differing in both physicochemical properties and AOB or AOA dominance of ammonia oxidization. Nitrification and N2O emissions were quantified under 10 Pa C2H2, 1-octyne, or 3,4-dimethylpyrazole phosphate (DMPP), and the community composition and abundance of AOA and AOB were assessed. Amendment of soil with DMPP had a comparable effect to 1-octyne on AOB activities, composition, and N2O emissions across soils. By blocking AOB activity, both DMPP and 1-octyne supported higher rates of AOA growth in both the acid and alkaline soils. Approximately, 50% of ammonia-oxidizing activity in the acid soil was attributed to AOB activity by phylotypes affiliated with Nitrosomonas communis. When AOB were blocked by DMPP or 1-octyne across all soils, recovery of AOA communities was associated with the increased abundance of Nitrososphaera viennesis.
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Acknowledgements
We thank Jipeng Luo for the helpful discussion for data analysis, and the gratitude is owned to Alin Song for the assistance of soil sampling. We are also grateful to the two anonymous reviewers and the chief editor for their instructive comments and suggestions.
Funding
This work was jointly supported by the grants from the National Natural Science Foundation of China (Approved No. 31800418), National Key Research and Development Program of China (2017YFD0200707 & 2017YFD0200102), and the Fundamental Research Funds for the Central Universities (2019FZJD007).
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Yin, C., Fan, X., Chen, H. et al. 3, 4-Dimethylpyrazole phosphate is an effective and specific inhibitor of soil ammonia-oxidizing bacteria. Biol Fertil Soils 57, 753–766 (2021). https://doi.org/10.1007/s00374-021-01565-1
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DOI: https://doi.org/10.1007/s00374-021-01565-1