Abstract
Drought stress, at different growth stages, causes substantial yield loss in major crops, thus, threatening global food security. Enhancement of drought stress tolerance in plants has been reported with the sole application of silicon (Si) and Zinc (Zn). However, the synergistic effects of their combined application and the exact mechanisms through which they enhance drought tolerance are still not fully understood. Therefore, the present study investigated the potential of Si and Zn to ameliorate drought stress in Eruca sativa (L.) and associated morphological and physio-biochemical mechanisms. The seeds were soaked in individual or combined Si (3 mM, from calcium silicate) and Zn (10 mM, from zinc sulfate) solutions for 8 h and 12 h. Water-primed and unprimed seeds were kept as control. Later, primed seeds were grown under various levels of drought viz. control (100% field capacity, FC) and 50% FC. Results revealed that drought stress significantly reduced growth traits, photosynthetic pigments, and reactive oxygen species and decreased antioxidative enzymes activities. Seed priming with Si and Zn increased seedling lengths, and their fresh and dry weights under drought stress with significantly higher values for the combined application of both. Moreover, Si and Zn-treated plants also showed a significant increase in chlorophyll pigments, i.e., chlorophyll and carotenoid contents in drought-exposed seedlings. Seed priming treatments improved drought-induced oxidative stress tolerance associated with higher activities of enzymatic and non-enzyme antioxidant activities and soluble sugar and proline contents. The results suggested that the combined application of Si and Zn serves as an appropriate treatment to increase the seedling growth, and physiological and biochemical attributes of E. sativa under drought stress conditions.
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References
Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126. https://doi.org/10.1016/S0076-6879(84)05016-3
Afrayeem SM, Chaurasia A (2017) Effect of zinc oxide nanoparticles on seed germination and seed vigour in chilli (Capsicum annuum L.). J Pharmacogn Phytochem 6:1564–1566. https://doi.org/10.22271/phyto.2017.v6.i5s.1847
Ahmad PMA, Ahanger P, Alam MN, Alyemeni MA (2018) Silicon (Si) supplementation alleviates NaCl toxicity in mung bean [Vigna radiata (L.) Wilczek] through the modifications of physio-biochemical attributes and key antioxidant enzymes. J Plant Growth Regul 38:70–82
Ahmed M, Qadeer U, Fayyaz-ul-Hassan Fahad S, Jatoi W, Duangpan S, Ahmad S (2020) Abiotic stress tolerance in wheat and the role of silicon: an experimental evidence. In: Hasanuzzaman M, Hakeem KR, Nahar K, Alharby HF, Fujita M (eds) Silicon in agriculture: from theory to practice. Springer, Berlin, pp 309–330. https://doi.org/10.1007/978-981-15-0025-1_22
Ahmad A, Aslam Z, Naz M, Hussain S, Javed T, Aslam S, Raza A, Ali HM, Siddiqui MH, Salem MZ, Hano C (2021) Exogenous salicylic acid-induced drought stress tolerance in wheat (Triticum aestivum L.) grown under hydroponic culture. PLoS ONE 16:0260556. https://doi.org/10.1371/journal.pone.0260556
Ahmad N, Virk AL, Hussain S et al (2022a) Integrated application of plant bioregulator and micronutrients improves crop physiology, productivity and grain biofortification of delayed sown wheat. Environ Sci Pollut Res 29:52534–52543. https://doi.org/10.1007/s11356-022-19476-5
Ahmad A, Aslam Z, Javed T, Hussain S, Raza A, Shabbir R, Mora-Poblete F, Saeed T, Zulfiqar F, Ali MM et al (2022b) screening of wheat (Triticum aestivum L.) genotypes for drought tolerance through agronomic and physiological response. Agronomy 12(2):287. https://doi.org/10.3390/agronomy12020287
Al-Quraan NA, Al-Ajlouni ZI, Qawasma NF (2021) Physiological and biochemical characterization of the Gaba shunt pathway in pea (Pisum sativum L.) seedlings under drought stress. Horticulturae. 7:125. https://doi.org/10.3390/horticulturae7050125
Alzahrani Y, Kusvuran A, Alharby HF, Kusvuran S, Rady MM (2018) The defensive role of silicon in wheat against stress conditions induced by drought, salinity or cadmium. Ecotoxicol Environ Saf 154:187–196. https://doi.org/10.1016/j.ecoenv.2018.02.035
Anjum SA, Tanveer M, Ashraf U et al (2016) Effect of progressive drought stress on growth, leaf gas exchange, and antioxidant production in two maize cultivars. Environ Sci Pollut Res 23:17132–17141. https://doi.org/10.1007/s11356-016-7216-3
Arslan MA, Rizwan R, Iqbal H, Arslan H, Adrees M, Zia ur Rehman M, Rizwan M, Shafaqat A (2022) Effect of different seed priming agents on chromium accumulation, oxidative defense, glyoxalase system and mineral nutrition in canola (Brassica napus L.) cultivars. Environ Pollut 309:119769. https://doi.org/10.1016/j.envpol.2021.119769
Avila RG, Magalhães PC, Silva EMD, Souza KRD, Campos CN, Alvarenga AAD, Souza TCD (2021) Application of silicon to irrigated and water deficit sorghum plants increases yield via the regulation of primary, antioxidant, and osmoregulatory metabolism. Agric Water Manag. 255:107004. https://doi.org/10.1016/j.agwat.2021.107004
Banerjee S, Islam J, Mondal S, Saha A, Saha B, Sen A (2023) Proactive attenuation of arsenic-stress by nano-priming: zinc oxide nanoparticles in Vigna mungo (L.) Hepper trigger antioxidant defense response and reduce root-shoot arsenic translocation. J Hazard Mater 446:130735. https://doi.org/10.1016/j.jhazmat.2022.130735
Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207. https://doi.org/10.1007/BF00018060
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287. https://doi.org/10.1016/0003-2697(71)90370-8
Begum N, Akhtar K, Ahanger MA, Iqbal M, Wang PP, Mustafa NS, Zhang LX (2021) Arbuscular mycorrhizal fungi improve growth, essential oil, secondary metabolism, and yield of tobacco (Nicotiana tabacum L.) under drought stress conditions. Environ Sci Pollut Res 28:45276–45295
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254
Bukhari MA, Sharif MS, Ahmad Z, Barutular C, Afzal M, Sabagh AE (2020) Silicon mitigates the adverse effect of drought in canola (Brassica napus L.) through promoting the physiological and antioxidants activity. SILICON 13:3717–3826
Cakmak I (1994) Activity of ascorbate-dependent H O-scavenging enzymes and leaf chlorosis are enhanced in magnesium- and potassium-deficient leaves, but not in phosphorus-deficient leaves. J Exp Bot 45:1259–1266. https://doi.org/10.1093/jxb/45.9.1259
Chakma R, Saekong P, Biswas A, Ullah H, Datta A (2021) Growth, fruit yield, quality, and water productivity of grape tomato as affected by seed priming and soil application of silicon under drought stress. Agric Water Manag 256:107055. https://doi.org/10.1016/j.agwat.2021.107055
Daryanto S, Wang L, Jacinthe PA (2022) Global synthesis of drought effects on cereal, legume, tuber and root crops production: a review. Agric Water Manag 179:18–33. https://doi.org/10.1016/j.agwat.2016.08.022
Dehghanipoodeh S, Ghobadi C, Baninasab B, Gheysari M, Bidabadi SS (2018) Effect of silicon on growth and development of strawberry under water deficit conditions. Hortic Plant J 4:226–232. https://doi.org/10.1016/j.hpj.2018.10.004
Dixit V, Pandey V, Shyam R (2001) Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad)1. J Exp Bot 52:1101–1109. https://doi.org/10.1093/jexbot/52.358.1101
Fatima A, Husain T, Suhel M et al (2022) Implication of nitric oxide under salinity stress: the possible interaction with other signaling molecules. J Plant Growth Regul 41:163–177. https://doi.org/10.1007/s00344-020-10255-5
García-López JI, Niño-Medina G, Olivares-Sáenz E et al (2019) Foliar application of zinc oxide nanoparticles and zinc sulfate boosts the content of bioactive compounds in habanero peppers. Plants 8:254. https://doi.org/10.3390/plants8070254
Granaz SK, Baksh G, Zahra N, Hafeez MB, Raza A, Samad A, Nizar M, Wahid A (2022) Foliar application of thiourea, salicylic acid, and kinetin alleviate salinity stress in maize grown under etiolated and de-etiolated conditions. Discover Food 2:1–14. https://doi.org/10.3390/discoverfoods2010001
Hameed A, Farooq T, Hameed A, Sheikh MA (2021) Silicon-mediated priming induces acclimation to mild water-deficit stress by altering physio-biochemical attributes in wheat plants. Front Plant Sci 12:625541. https://doi.org/10.3389/fpls.2021.625541
Hamilton PB, Van-Slyke DD (1943) Amino acid determination with ninhydrin. J Biol Chem 150:231–233. https://doi.org/10.1016/S0021-9258(18)81355-5
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198. https://doi.org/10.1016/0003-9861(68)90654-1
Hodges DM, Nozzolillo C (1996) Anthocyanin and anthocyanoplast content of cruciferous seedlings subjected to mineral nutrient deficiencies. J Plant Physiol 47:749–754. https://doi.org/10.1016/S0176-1617(96)80260-8
Huang S, Fan TL, Liu MJ, Chen RH, Liang CT, Cheng WL, Chen YH, Xue S, Yang XM (2021) Effects of plastic film residues on photophysiological characteristics and biomass accumulation of soybean (Glycine max). Chin J Ecol Agric 29:979–990. https://doi.org/10.12173/j.cnki.2095-8353.20200327-099
Husain T, Fatima A, Suhel M, Singh S, Sharma A, Prasad SM, Singh VP (2020) A brief appraisal of ethylene signaling under abiotic stress in plants. Plant Signal Behav 15:9. https://doi.org/10.1080/15592324.2020.1782051
Husain T, Suhel M, Prasad SM, Singh VP (2022) Ethylene and hydrogen sulphide are essential for mitigating hexavalent chromium stress in two pulse crops. Plant Biol J 24:652–659. https://doi.org/10.1111/plb.13324
Hussain MH, Biradar CH (2021) Experimental investigation of performance and combustion characteristics of a diesel engine fueled with Eruca sativa (Taramira) biodiesel–diesel blend. In: Chakrabarty A, Kumar S, Nagar AK (eds) Advances in industrial automation and smart manufacturing. Springer, Singapore, pp 879–893. https://doi.org/10.1007/978-981-16-2916-2_78
Hussain HA, Men S, Hussain S, Chen Y, Ali S, Zhang S, Zhang K, Li Y, Xu Q, Liao C et al (2019a) Interactive effects of drought and heat stresses on morpho-physiological attributes, yield, nutrient uptake and oxidative status in maize hybrids. Sci Rep 9:3890. https://doi.org/10.1038/s41598-019-40734-9
Hussain S, Hussain S, Khaliq A, Ali S, Khan I (2019b) Physiological, biochemical, and molecular aspects of seed priming. In: Hasanuzzaman M, Fotopoulos V (eds) Priming and pretreatment of seeds and seedlings. Springer, Singapore. https://doi.org/10.1007/978-981-13-8625-1_3
Hussain S, Hussain S, Qadir T, Khaliq A, Ashraf U, Parveen A, Saqib M, Rafiq M (2019c) Drought stress in plants: an overview on implications, tolerance mechanisms and agronomic mitigation strategies. Plant Sci Today 6:389–402. https://doi.org/10.14719/pst.2019.6.4.389
Hussain S, Ali B, Saqib M (2021) Seed priming to enhance salt and drought stress tolerance in plants: advances and prospects. In: Shanker AR, Anand A, Shanker C, Maheswari M (eds) Climate change and crop stress: molecules to ecosystems. Academic Press, New York, pp 441–464. https://doi.org/10.1016/B978-0-12-816091-6.00012-2
Hussain S, Hafeez MB, Azam R et al (2023) Deciphering the role of phytohormones and osmolytes in plant tolerance against salt stress: implications, possible cross-talk, and prospects. J Plant Growth Regul. https://doi.org/10.1007/s00344-023-11070-4
Hussan MU, Saleem MF, Hafeez MB, Khan S, Hussain S, Ahmad N, Ramzan Y, Nadeem M (2022) Impact of soil applied humic acid, zinc and boron supplementation on the growth, yield and zinc translocation in wheat. Asian J. Agric. Biol. 2022(1):202102080. https://doi.org/10.35495/ajab.2021.02.080
Ibrahim MU, Khaliq A, Hussain S, Murtaza G (2022) Sorghum water extract application mediates anti-oxidant defense and confers drought stress tolerance in wheat. J Plant Growth Regul 41:863–874. https://doi.org/10.1007/s00344-021-10359-w
Iqbal M, Rasheed R, Ashraf MY et al (2018) Exogenously applied zinc and copper mitigate salinity effect in maize (Zea mays L.) by improving key physiological and biochemical attributes. Environ Sci Pollut Res 25:23883–23896. https://doi.org/10.1007/s11356-018-2383-6
Jan M, Anwar-Ul-Haq M, Javed T, Hussain S, Ahmad I, Sumrah MA, Iqbal J, Babar BH, Hafeez A, Aslam M, Akbar MT (2023) Response of contrasting rice genotypes to zinc sources under saline conditions. Phyton 92:1361–1375
Jia X, Mao K, Wang P, Wang Y, Jia XM, Huo LQ, Sun X, Che RM, Gong XQ, Ma FW (2021) Overexpression of MdATG8i improves water use efficiency in transgenic apple by modulating photosynthesis, osmotic balance, and autophagic activity under moderate water deficit. Hortic Res 8:855–869
Julkenen-Titto R (1985) Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. Agric Food Chem 33:213–217. https://doi.org/10.1021/jf00063a018
Kamal NM, Gorafifi YSA, Abdelrahman M, Abdellatef E, Tsujimoto H (2019) Stay-green trait: a prospective approach for yield potential, and drought and heat stress adaptation in globally important cereals. Int J Mol Sci 20:5837. https://doi.org/10.3390/ijms20235837
Kandil E, Lamlom S, Gheith E, Javed T, Ghareeb R, Abdelsalam N, Hussain S (2023) Biofortification of maize growth, productivity and quality using nano-silver, silicon and zinc particles with different irrigation intervals. J Agric Sci. https://doi.org/10.1017/S0021859623000345
Kausar A, Hussain S, Javed T, Zafar S, Anwar S, Hussain S, Zahra N, Saqib M (2023) Zinc oxide nanoparticles as potential hallmarks for enhancing drought stress tolerance in wheat seedlings. Plant Physiol Biochem 195:341–350
Khan MT, Ahmed S, Shah AA, Noor Shah A, Tanveer M, El-Sheikh MA, Siddiqui MH (2021) Influence of zinc oxide nanoparticles to regulate the antioxidants enzymes, some os-molytes and agronomic attributes in Coriandrum sativum L. grown under water stress. Agronomy. 10:2004–06. https://doi.org/10.3390/agronomy11102004
Kim YH, Khan AL, Waqas M, Lee IJ (2017) Silicon regulates antioxidant activities of crop plants under abiotic-induced oxidative stress: a review. Front Plant Sci 8:510
Li GJ, Liu YH, Wu XH, Zhu ZJ, Wang BG, Lu ZF (2007) Effects of exogenous silicon on reactive oxygen species metabolism of Asparagus bean seedlings under rust stress. Acta Hortic Sin 34:1207–1212
Li SX, Liu WG, Gao Y, Liu T, Zhou T, Du Y, Yang H, Zhang H, Liu JD, Yang W (2018) Effects of silicon on plant growth and photosynthetic characteristics of soybean seedlings under artificial shade stress. Sci Agric Sin 51:3663–3672
Li SX, Liu WG, Gao Y, Liu T, Zhou T, Du Y, Yang H, Zhang H, Liu JD, Yang WY, Ahmad P, Ahanger MA, Alyemeni MN, Wijaya L, Egamberdieva D, Bhardwaj R, Ashraf M (2021) Zinc application mitigates the adverse effects of NaCl stress on mustard [Brassica juncea (L.) Czern & Coss] through modulating compatible organic solutes, antioxidant enzymes, and flavonoid content. J Plant Interact 12:429–437. https://doi.org/10.1080/17429145.2017.1355222
Lichtenthaler HK (1987) Chlorophylls + carotenoid determination. Karlsruhe Institute of Technology, Karlsruhe. https://doi.org/10.13140/RG.2.2
Ma D, Sun D, Wang C, Ding H, Qin H, Hou J et al (2017) Physiological responses and yield of wheat plants in zinc-mediated alleviation of drought stress. Front Plant Sci 8:860. https://doi.org/10.3389/fpls.2017.00860
Mahmood U, Li X, Fan Y, Chang W, Niu Y, Li J, Qu C, Lu K (2022) Multi-omics revolution to promote plant breeding efficiency. Front Plant Sci 13:1062952
Mahmood U, Li X, Qian M, Fan Y, Yu M, Li S, Shahzad A, Qu C, Li J, Liu L, Lu K (2023) Comparative transcriptome and co-expression network analysis revealed the genes associated with senescence and polygalacturonase activity involved in pod shattering of rapeseed. Biotechnol Biofuels Bioprod 16(1):1–17
Malik MA, Wani AH, Mir SH, Rehman IU, Tahir I, Ahmad P (2021) Elucidating the role of silicon in drought stress tolerance in plants. Plant Physiol Biochem 165:187–195. https://doi.org/10.1016/j.plaphy.2021.06.006
Mamrutha HM, Singh R, Sharma D, Venkatesh K, Pandey GC, Kumar R, Tiwari R, Sharma I (2019) Physiological and molecular basis of abiotic stress tolerance in wheat. In: Rajpal VR, Sehgal D, Kumar A, Raina SN (eds) Genetic enhancement of crops for tolerance to abiotic stress: mechanisms and approaches, vol I. Springer, Cham
Mazhar M, Ishtiaq ChM, Hussain I, Parveen D, Bhatti K, Thind S, Ajaib M, Maqbool M, Sardar T, Muzammil K, Nasir N (2022a) Seed nano-priming with zinc oxide nanoparticles in rice mitigates drought and enhances agronomic profile. PLoS ONE 17:e0264967. https://doi.org/10.1371/journal.pone.0264967
Mazhar M, Ishtiaq ChM, Maqbool M, Akram R (2022b) Seed priming with zinc oxide nanoparticles improves growth, osmolyte accumulation, antioxidant defence and yield quality of water-stressed mung bean plants. Arid Land Res Manage. https://doi.org/10.1080/15324982.2022.2132547
McDowell NG, Sapes G, Pivovaroff A, Adams HD, Allen CD, Anderegg WRL, Arend M, Breshears DD, Brodribb T, Choat B et al (2022) Mechanisms of woody-plant mortality under rising drought, CO2 and vapour pressure deficit. Nat Rev Earth Environ 3:294–308
Mehmood M, Khan I, Chattha MU, Hussain S, Ahmad N, Aslam MT, Hafeez MB, Hussan M, Hassan MU, Nawaz M, Iqbal MM (2021) Thiourea application protects maize from drought stress by regulating growth and physiological traits. Pak J Sci 73:355. https://doi.org/10.1155/2021/6650383
Merwad AM, Desoky EM, Rady MM (2018) Response of water deficit-stressed Vigna unguiculata performances to silicon, proline or methionine foliar application. Sci Hortic 228:132–144. https://doi.org/10.1016/j.scienta.2017.09.047
Moller IM, Jensen PE, Hansson A (2007) Oxidative modifications to cellular components in plants. Annu Rev Plant Biol 58:459–481. https://doi.org/10.1146/annurev.arplant.58.032806.103946
Mubarik MS, Khan SH, Sajjad M et al (2021) A manipulative interplay between positive and negative regulators of phytohormones: a way forward for improving drought tolerance in plants. Physiol Plantarum 172(2):1269–1290. https://doi.org/10.1111/ppl
Mukherjee SP, Choudhuri MA (1983) Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:166–170. https://doi.org/10.1111/j.1399-3054.1983.tb04162.x
Nawaz A, Farooq J, Hussain M, Farooq M, Cheema MA, Wahid MA (2013) Stay green character at grain filling ensures resistance against terminal drought in wheat. Int J Agric Biol s15:1272–1276
Naz M, Hussain S, Ashraf I, Farooq M (2023) Exogenous application of proline and phosphorus help improving maize performance under salt stress. J Plant Nutr 46(10):2342–2350. https://doi.org/10.1080/01904167.2022.2155541
Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J Biol Chem 153:375–380. https://doi.org/10.1016/s0021-9258(19)52451-6
Ola MP, Jakhar ML, Gothwal DK, Ahmad S, Ram M (2021) Genotypic × environment interaction and stability analysis for yield and yield attributes in taramira (Eruca sativa Mill.). Int J Environ Clim Change 11:1–11. https://doi.org/10.9734/ijecc/2021/v11i230379
Parveen A, Liu W, Hussain S, Asghar J, Perveen S, **ong Y (2019) Silicon priming regulates morpho-physiological growth and oxidative metabolism in maize under drought stress. Plants 8:431
Patel MD, Fatnani AK, Parida AK (2021) Silicon-induced mitigation of drought stress in peanut genotypes (Arachis hypogaea L.) through ion homeostasis, modulations of antioxidative defense system, and metabolic regulations. Plant Physiol Biochem 166:290–313. https://doi.org/10.1016/j.plaphy.2021.11
Rangani J, Panda A, Patel M, Parida AK (2018) Regulation of ROS through proficient modulations of antioxidative defense system maintains the structural and functional integrity of photosynthetic apparatus and confers drought tolerance in the facultative halophyte Salvadora persica L. J Photochem Photobiol B Biol 189:214–233
Reddy AR, Chaitanya KV, Vivekanandan M (2004) Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161:1189–1202. https://doi.org/10.1016/j.jplph.2004.01.013
Ribarova F, Atanassova M, Marinova D, Ribarova F, Atanassova M (2005) Total phenolics and flavonoids in Bulgarian fruits and vegetables. JU Chem Metal 40:255–260. https://doi.org/10.2298/JSC0504255R
Rocha JR, Prado RDM, Piccolo MDC (2022) Mitigation of water deficit in two cultivars of Panicum maximum by the application of silicon. Water Air Soil Pollut 233(3):63. https://doi.org/10.1007/s11270-022-5492-y
Sabagh AEL, Hossain A, Barutçular C, Iqbal MA, Islam MS, Fahad S, Sytar O, ÇIĞ F, Meena RS, Erman M (2020) Consequences of salinity stress on the quality of crops and its mitigation strategies for sustainable crop production: an outlook of arid and semi-arid regions. In: Environment, climate, plant and vegetation growth. Springer, Cham, pp 503–533. https://doi.org/10.1007/978-3-030-38279-3_21
Sadati SYR, Godehkahriz SJ, Ebadi A, Sedghi M (2022) Zinc oxide nanoparticles enhance drought tolerance in wheat via physio-biochemical changes and stress genes expression. Iran J Biotechnol 2:3027. https://doi.org/10.30498/ijb.2021.2636
Saddiq MS, Wang X, Iqbal S et al (2021) Effect of water stress on grain yield and physiological characters of quinoa genotypes. Agronomy 11:1934. https://doi.org/10.3390/agronomy11101934
Sadeghizadeh M, Zarea M (2022) Effects of seed priming with zinc on germination, nursery seedling growth and paddy fields yield of two rice (Oryza sativa L.) cultivars. J Crop Sci Biotechnol 25:1–13
Sahoo S, Borgohain P, Saha B, Moulick D, Tanti B, Panda SK (2019) Seed priming and seedling pre-treatment induced tolerance to drought and salt stress: recent advances. In: Hasanuzzaman M, Fotopoulos V (eds) Priming and pretreatment of seeds and seedlings. Springer, Singapore. https://doi.org/10.1007/978-981-13-8625-1_12
Shabbir R, Singhal RK, Mishra UN, Chauhan J, Javed T, Hussain S, Kumar S, Anuragi H, Lal D, Chen P (2022) Combined abiotic stresses: challenges and potential for crop improvement. Agronomy 12(11):2795. https://doi.org/10.3390/agronomy12112795
Shahzad A, Qian M, Sun B, Mahmood U, Li S, Fan Y, Chang W, Dai L, Zhu H, Li J, Qu C (2021) Genome-wide association study identifies novel loci and candidate genes for drought stress tolerance in rapeseed. Oil Crop Science 6(1):12–22
Singh S, Husain T, Kushwaha BK, Mohd Suhel A, Fatima V, Mishra SK, Singh JA, Bhatt M, Rai SM, Prasad NK, Dubey DK, Chauhan DK, Tripathi V, Fotopoulos, Singh VP (2021) Regulation of ascorbate-glutathione cycle by exogenous nitric oxide and hydrogen peroxide in soybean roots under arsenate stress. J Hazard Mater. https://doi.org/10.1016/j.jhazmat.2020.123686
Suhel M, Husain T, Pandey A et al (2023a) An appraisal of ancient molecule GABA in abiotic stress tolerance in plants, and its crosstalk with other signaling molecules. J Plant Growth Regul 42:614–629. https://doi.org/10.1007/s00344-022-10610-8
Suhel M, Husain T, Prasad SM et al (2023b) GABA requires nitric oxide for alleviating arsenate stress in tomato and brinjal seedlings. J Plant Growth Regul 42:670–683. https://doi.org/10.1007/s00344-022-10576-7
Talha J, Rubab S, Sadam H, Asad NM, Irsa E, Moaaz AM, Sunny A, Fathy YA (2022) Nanotechnology for endorsing abiotic stresses: a review on the role of nanoparticles and nanocompositions. Funct Plant Biol. https://doi.org/10.1071/FP22092
Tuiwong P, Lordkaew S, Veeradittakit J, Jamjod S, Prom-u-thai C (2022) Efficacy of nitrogen and zinc application at different growth stages on yield, grain zinc, and nitrogen concentration in rice. Agronomy 12:2093
Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. Plant Sci 151:59–66. https://doi.org/10.1016/s0168-9452(99)00197-1
Wagaw K (2019) Isolation and screening of ACC deaminase-producing microbes for drought stress management in crops. Acad Res J Agri Sci Res 7:87–99
Wang Y, Zhang B, Jiang D, Chen G (2019) Silicon improves photosynthetic performance by optimizing thylakoid membrane protein components in rice under drought stress. Environ Exp Bot 158:117–124. https://doi.org/10.1016/j.envexpbot.2018.11.010
Wenneck GS, Saath R, Rezende R et al (2022) Silicon application increases water productivity in cauliflower under sub-tropical condition. Agric Res 11:103–113
Yadav B, Jogawat A, Lal SK, Lakra N, Mehta S, Shabek N, Narayan OP (2021) Plant mineral transport systems and the potential for crop improvement. Planta 253:1–30. https://doi.org/10.1007/s00425-021-03630-5
Zafar S, Perveen S, Kamran Khan M et al (2022) Effect of zinc nanoparticles seed priming and foliar application on the growth and physio-biochemical indices of spinach (Spinacia oleracea L.) under salt stress. PLoS ONE 17:0263194. https://doi.org/10.1371/journal.pone.0263194
Zafar M, Ahmed S, Munir MK, Zafar N, Saqib M, Sarwar MA, Iqbal S, Ali B, Akhtar N, Ali B, Hussain S (2023) Application of zinc, iron and boron enhances productivity and grain biofortification of mungbean. Phyton 92:983–999
Zhang X, Zhang W, Lang D, Cui J, Li Y (2018) Silicon improves salt tolerance of Glycyrrhiza uralensis Fisch by ameliorating osmotic and oxidative stresses and improving phytohormonal balance. Environ Sci Pollut Res 25:25916–25932. https://doi.org/10.1007/s11356-018-2609-9
Zhang WJ, Zhang XJ, Lang DY, Li M (2020) Silicon alleviates salt and drought stress of Glycyrrhiza uralensis plants by improving photosynthesis and water status. Biol Plant 64:302–313. https://doi.org/10.1007/s10535-020-00245-5
Zulfiqar U, Maqsood M, Hussain S (2020) Biofortification of rice with iron and zinc: progress and prospects. In: Roychoudhury A (ed) Rice research for quality improvement: genomics and genetic engineering, vol 2: nutrient biofortification and herbicide and biotic stress resistance in rice. Springer, Singapore, pp 605–627
Acknowledgements
The authors would like to extend their sincere appreciation to the Researchers Supporting Project Number (RSP2023R347), King Saud University, Riyadh, Saudi Arabia.
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This work was supported by the Researchers Supporting Project number (RSP2023R347), King Saud University, Riyadh, Saudi Arabia.
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Conceptualization, IH and AY; methodology, AY; software, AY; validation, IH, AY and AN; formal analysis, MAA; investigation, IH; resources, IH; data curation, IH; writing—original draft preparation, IH; writing—review and editing, SH; visualization, MAA; supervision, AN; project administration, IH; funding acquisition, RMB. All authors have read and agreed to the published version of the manuscript.
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Hussain, I., Ayub, A., Nayab, A. et al. Exogenous Application of Silicon and Zinc Attenuates Drought Tolerance in Eruca sativa L. Through Increasing Chlorophyll Pigments, Osmoprotectants, and Modulating Defense Mechanisms. J Plant Growth Regul (2023). https://doi.org/10.1007/s00344-023-11116-7
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DOI: https://doi.org/10.1007/s00344-023-11116-7