Log in

Identification and characterization of microbial agents in a bloom of floating microorganisms in the Hailang River, China in 2006

  • Published:
Chinese Journal of Oceanology and Limnology Aims and scope Submit manuscript

Abstract

In February 2006, a large amount of unknown floating microorganisms appeared in the Hailang River (HR) in the City of Hailin, Heilongjiang Province, China. The microorganisms caused the river water fouling and threatened the clean water supply of the city. To identify the unknown floating organisms, morphological and histological inspection, PCR, cloning and sequence analysis were conducted. The results revealed that the unknown floating organisms in the river were a novel species that likely belonged to oomycetes in Saprolegniales. The organisms were named Saprolegnia sp. HL0602.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Chandra R, Singh S, Raj A. 2006. Seasonal bacteriological analysis of Gola river water contaminated with pulppaper mill waste in Uttaranchal, India. Environ. Monit. Assess, 118(1–3): 393–4 016.

    Article  Google Scholar 

  • Gunnison D. 1999. Evaluating Microbial Pathogens in Reservoirs. Water Quality Technical Notes Collection (WQTN PD-03). US Army Engineer Research and Development Center, Vicksburg, MS.

    Google Scholar 

  • Hageskal G, Gaustad P, Heier B, Skaar I.2007. Occurrence of moulds in drinking water. J. Appl. Microbiol., 102(3): 774–780.

    Article  Google Scholar 

  • Hopfer R L, Walden P, Setterquist S, Highsmith W E. 1993. Detection and differentiation of fungi in clinical specimens using polymerase chain reaction (PCR) amplification and restriction enzyme analysis. J. Med. Vet. Mycol., 31(1): 65–75.

    Article  Google Scholar 

  • Kataria H C, Iqbal S A, Shandilya A K. 1997. MPN of total coliform as pollution indicator in Halali river water of Madhya Pradesh India. Pollut. Res., 16(1): 255–257.

    Google Scholar 

  • Kittigul L, Uthaisin A, Ekchaloemkiet S, Utrarachkij F, Luksamijarulkul P. 2006. Detection and characterization of hepatitis A virus in water samples in Thailand. J. Appl. Microbiol., 100(6): 1 318–1 323.

    Article  Google Scholar 

  • Marcheggiani S, Iaconelli M, D’angelo A, Pierdominici E, La Rosa G, Muscillo M, Equestre M, Mancini L. 2008. Microbiological and 16S rRNA analysis of sulphite-reducing clostridia from river sediments in central Italy. BMC Microbiol., 8(8): 171.

    Article  Google Scholar 

  • Nechwatal J, Wielgoss A, Mendgen K. 2008. Diversity, host, and habitat specificity of oomycete communities in declining reed stands (Phragmites australis) of a large freshwater lake. Mycol. Res., 112(3): 689–696.

    Article  Google Scholar 

  • Ramteke P W, Pathak S P, Bhattacherjee J W, Gopal K, Mathur N. 1994. Evaluation of the presence-absence (P-A) test: A simplified bacteriological test for detecting coliform in rural drinking water of India. Environ. Monit. Assess., 33(1): 53–59.

    Article  Google Scholar 

  • Reiss E, Tanaka K, Bruker G, Chazalet V, Coleman D, Debeaupuis J P, Hanazawa R, Latgé J P, Lortholary J, Makimura K, Morrison CJ, Murayama SY, Naoe S, Paris S, Sarfati J, Shibuya K, Sullivan D, Uchida K, Yamaguchi H. 1998. Molecular diagnosis and epidemiology of fungal infections. Med. Mycol., 36(Suppl 1): 249–257.

    Google Scholar 

  • Savichtcheva O, Okabe S. 2009. Qualitative and quantitative estimation of host-specific fecal pollution using Bacteroides-Prevotella 16S rRNA genetic markers by T-RFLP and real-time PCR analyses. Water Sci. Technol., 59(9): 1 831–1 840.

    Google Scholar 

  • Shang S, Chen Z, Yu X. 2001. Detection of bacterial DNA by PCR and reverse hybridization in the 16S rRNA gene with particular reference to neonatal septicemia. Acta Paediatr., 90(2): 179–183.

    Article  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S. 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol., 24(8): 1 596–1 599.

    Article  Google Scholar 

  • White T J, Bruns T, Lee S B, Taylor J W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Academic Press, San Diego.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Weijun Chen  (陈唯军).

Additional information

Supported by Infectious Diseases Special Project, Minister of Health of China (2008ZX10004-001)

All the authors contributed equally.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wu, W., Wang, J., Liu, L. et al. Identification and characterization of microbial agents in a bloom of floating microorganisms in the Hailang River, China in 2006. Chin. J. Ocean. Limnol. 28, 836–841 (2010). https://doi.org/10.1007/s00343-010-9060-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00343-010-9060-z

Keyword

Navigation