Abstract
Key message
The interaction network and pathway map uncover the potential crosstalk between sugar and hormone metabolisms as a possible reason for leaf senescence in P. ternata.
Abstract
Pinellia ternata, an environmentally sensitive medicinal plant, undergoes leaf senescence twice a year, affecting its development and yield. Understanding the potential mechanism that delays leaf senescence could theoretically decrease yield losses. In this study, a typical senescent population model was constructed, and an integrated analysis of transcriptomic and metabolomic profiles of P. ternata was conducted using two early leaf senescence populations and two stay-green populations. The result showed that two key gene modules were associated with leaf senescence which were mainly enriched in sugar and hormone signaling pathways, respectively. A network constructed by unigenes and metabolisms related to the obtained two pathways revealed that several compounds such as d-arabitol and 2MeScZR have a higher significance ranking. In addition, a total of 130 hub genes in this network were categorized into 3 classes based on connectivity. Among them, 34 hub genes were further analyzed through a pathway map, the potential crosstalk between sugar and hormone metabolisms might be an underlying reason of leaf senescence in P. ternata. These findings address the knowledge gap regarding leaf senescence in P. ternata, providing candidate germplasms for molecular breeding and laying theoretical basis for the realization of finely regulated cultivation in future.
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Data availability
The raw data that support the findings of this study are available in National Center for Biotechnology Information (NCBI, https://www.ncbi.nlm.nih.gov). The associated BioProject, BioSample, and SRA numbers are PRJNA1056149, SAMN39090724, and SRR27334041, respectively. These data were released on October 1, 2024.
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Funding
This work was supported by grants from the Scientific and Technological Innovation project of China Academy of Chinese Medical Sciences (CACMS Innovation Fund CI2021A04106, CI2021A03910), the Fundamental Research Funds for the Central public welfare research institutes of China (ZXKT21026, ZZ15-YQ-033, ZXKT23004), the Major Special Project of Scientific and Technological Cooperation of Bijie City (2021–02), and Bijie Science and Technology Innovation Platform and Talent Team (2023–66)-Bijie Key Laboratory of Health Industry (BJZDSYS 2024–05).
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Jialei Chen: writing—original draft, investigation, data curation, formal analysis, resources, visualization; Jialu Wang: writing—original draft, formal analysis, validation, visualization; Li Liu: investigation, methodology, resources; Yifei Pei: visualization; Ziyi Liu: resources; Xue Feng: conceptualization, funding acquisition, validation, supervision, methodology, writing—review and editing; **wen Li: funding acquisition, supervision, writing—review and editing.
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Chen, J., Wang, J., Liu, L. et al. Transcriptomic and metabolomic profiling provide insight into the role of sugars and hormones in leaf senescence of Pinellia ternata. Plant Cell Rep 43, 125 (2024). https://doi.org/10.1007/s00299-024-03222-x
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DOI: https://doi.org/10.1007/s00299-024-03222-x