Abstract
Key message
OsiCRY2 is involved in light-regulated plant development and plays a role in regulating photomorphogenesis, plant height, flowering and most strikingly partial photomorphogenesis in dark.
Abstract
Cryptochrome 2 (CRY2), the blue/UV-A light photoreceptor in plants, has been reported to regulate photoperiod-dependent flowering and seedling photomorphogenesis (under low-intensity light). Among monocots, CRY2 has been reported from japonica rice, wheat, sorghum and barley. The two sub-species of rice, indica and japonica, exhibit a high degree of genetic variation and morphological and physiological differences. This article describes the characterization of CRY2 of indica rice (OsiCRY2). While the transcript levels of OsiCRY2 did not change significantly under blue light, its protein levels were found to decline with increased time duration under blue light. For phenotypic characterization, OsiCRY2 over-expression (OX) transgenics were generated in Oryza sativa Pusa Sugandh 2 (PS2) cultivar, a highly scented Basmati cultivar. The OsiCRY2OX transgenics displayed shorter coleoptiles and dwarfism than wild-type under blue light, white, and far-red light. Interestingly, even the dark-grown transgenics were shorter, concomitant with higher OsiCRY2 protein levels in transgenics than wild-type. Histological analysis revealed that the decrease in the length of the seedlings was due to a decrease in the length of the epidermal cells. The fully mature rice transgenics were shorter than the untransformed plants but flowered at the same time as wild-type. However, the OsiCRY2 Arabidopsis over-expressors exhibited early flowering by 10–15 days, indicating the potential and conservation of function of OsiCRY2. The whole-genome transcriptome profiling of rice transgenics revealed the differential up-regulation of several light-regulated genes in dark-grown coleoptiles. These data provide evidence that OsiCRY2 regulates photomorphogenesis, plant height, and flowering in indica rice.
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Data availability
The microarray data have been uploaded in Gene Expression Omnibus (GEO) database and its accession number is GSE200963.
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Acknowledgements
This research was funded by the Department of Biotechnology, Government of India (BT/PR12394/AGIII/103/891/2014) and the J.C. Bose National Fellowship to JPK by the Science and Engineering Research Board (SERB), Government of India. The authors also acknowledge the infrastructural support provided by the Department of Science and Technology (FIST and PURSE programmes), Government of India, and the University Grants Commission (UGC-SAP), New Delhi. The award of Senior Research Fellowship from the Council of Scientific and Industrial Research (CSIR), New Delhi, to PS, SM and SS is duly acknowledged.
Funding
Department of Biotechnology, Government of India, BT/PR12394/AGIII/103/891/2014, Council for Scientific and Industrial Research, India, J.C. Bose National Fellowship, SB/SR/JCB-13/2013, Jitendra P Khurana.
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Singh, S., Sharma, P., Mishra, S. et al. CRY2 gene of rice (Oryza sativa subsp. indica) encodes a blue light sensory receptor involved in regulating flowering, plant height and partial photomorphogenesis in dark. Plant Cell Rep 42, 73–89 (2023). https://doi.org/10.1007/s00299-022-02937-z
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DOI: https://doi.org/10.1007/s00299-022-02937-z