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Reclassification of Some Exiguobacterium Species Based on Genome Analysis

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The Exiguobacterium genus comprises Gram-stain-positive and facultatively anaerobic bacteria. Some Exiguobacterium species have previously shown significant high 16S rRNA gene sequence similarities with each other. This study evaluates the taxonomic classification of those Exiguobacterium species through comprehensive genome analysis. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values were determined for various Exiguobacterium species pairs. The ANI and dDDH values between Exiguobacterium enclense and Exiguobacterium indicum, Exiguobacterium aquaticum and Exiguobacterium mexicanum, Exiguobacterium soli and Exiguobacterium antarcticum, and Exiguobacterium sibiricum and Exiguobacterium artemiae were above the cut-off level (95–96% for ANI and 70% for dDDH) for species delineation. Based on the findings, we propose to reclassify Exiguobacterium enclense as a later heterotypic synonym of Exiguobacterium indicum, Exiguobacterium aquaticum as a later heterotypic synonym of Exiguobacterium mexicanum, Exiguobacterium soli as a later heterotypic synonym of Exiguobacterium antarcticum and Exiguobacterium sibiricum as a later heterotypic synonym of Exiguobacterium artemiae.

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Acknowledgements

Exiguobacterium soli DSM 22015T genome sequence data were produced by the US Department of Energy Joint Genome Institute http://www.jgi.doegov/ in collaboration with the user community. We thank Dr. Markus Göker (Leibniz Institute DSMZ) for permitting us to use Exiguobacterium soli DSM 22015T genome sequence.

Funding

This research was supported by the National Science and Technology Fundamental Resources Investigation Program of China (2021FY100900), Key Scientific and Technological Project of Heilongjiang Province of China (2021ZXJ03B05) and the Projects of the Heilongjiang Academy of Agricultural Sciences (CX23GG10). The author Shuang Wang was also supported by Introduction project of high-level talents in **njiang Uygur Autonomous Region. The author Syed Raziuddin Quadri extends his appreciation to the Deanship of Scientific Research at Northern Border University, Arar, Kingdom of Saudi Arabia for funding this research work through the project number NBU-FFR-2024–2046-03.

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Authors and Affiliations

  1. Heilongjiang Academy of Black Soil Conservation and Utilization/Heilongjiang Black Soil Conservation Engineering and Technology Research Center, Harbin, 150086, People’s Republic of China

    Shuang Wang, Pinjiao ** & Kangkang Wang

  2. State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, **njiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, People’s Republic of China

    Shuang Wang

  3. Instituto de Ciencias Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, Sede Talca, 3460000, Talca, Chile

    Manik Prabhu Narsing Rao

  4. Department of Microbiology, Maharani’s Science College for Women, Bangalore, 560001, India

    Awalagaway Dhulappa

  5. Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, Northern Border University, Arar, 91431, Kingdom of Saudi Arabia

    Syed Raziuddin Quadri

  6. Dengta City Development Reform Affairs Service Center, Dengta, 150499, People’s Republic of China

    Hui Qiao

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Contributions

MPNR supervised the study. SW designed the research and project outline. PJ, KW, AD and SRQ conducted the genome analysis. MPNR, HQ, PJ and KW revised the final manuscript. All authors read and approved the final manuscript.

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Correspondence to Manik Prabhu Narsing Rao.

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Wang, S., Dhulappa, A., Quadri, S.R. et al. Reclassification of Some Exiguobacterium Species Based on Genome Analysis. Curr Microbiol 81, 186 (2024). https://doi.org/10.1007/s00284-024-03735-4

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