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Effects of Voltage and Treatment Time of Pulsed Electric Field on Electroporation in Rhizoctonia solani

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Abstract

The pulsed electric field (PEF) of μs duration can induce electroporation by causing permanent damage to the membrane, leading to cell death. The microbe was treated by a homemade PEF generator instrument. The sterilization effect of PEF on the Rhizoctonia solani was observed by scanning electron microscope (SEM) and transmission electron microscope (TEM), and the leakage of the intracellular contents was measured with a conductometer and an ultraviolet spectrophotometer. The increases in the electrical conductivity and the optical density (OD) value indicated that the cell membrane was damaged, and the intracellular contents overflowed. As a result, according to our experimental conditions, the optimum condition was the high-pulsed electric voltage of 26 kV, and the treatment time was 4 min. It could be concluded that the PEF could damage the cell membrane, and the ratio of electroporation reached 100%, which provides a new method of killing R. solani efficiently.

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Data Availability

The datasets generated during and/or analyzed during the current study are not publicly available due to privacy reasons but are available from the corresponding author on reasonable request.

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Funding

His research was funded by the Central Universities (KJQN201623) and the National Key Research and Development Program of China(2017YFD0700800).

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Authors and Affiliations

  1. College of Engineering, Nan**g Agricultural University, Nan**g, 210031, China

    Xuebin Feng, Xueru Zhu, Mengyu Zhu, Yan Qian & Hua Li

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  1. Xuebin Feng
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  2. Xueru Zhu
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  3. Mengyu Zhu
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  4. Yan Qian
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  5. Hua Li
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Contributions

All authors contributed to the study’s conception and design. Material preparation, data collection, and analysis were performed by XZ, MZ, YQ, and HL. The first draft of the manuscript was written by XF and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Hua Li.

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Feng, X., Zhu, X., Zhu, M. et al. Effects of Voltage and Treatment Time of Pulsed Electric Field on Electroporation in Rhizoctonia solani. Curr Microbiol 81, 58 (2024). https://doi.org/10.1007/s00284-023-03564-x

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