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Obtaining Osmo-resistant Mutants in Nitrogen-Fixing Bacteria Isolated from Saline Soils

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Abstract

Annually, about, more than 7% of the Earth’s land area becomes inappropriate for agriculture subsequently of salinization and desertification. Biofertilizers based on halophilic nitrogen-fixing bacteria can restore saline soils and stimulate plant growth, having a positive effect on germination, development of stems and roots, and fruiting. The aim of this work was to obtain osmo-resistant (Osm-r) nitrogen-fixing mutants isolated from saline soils of Armenia and selection of the best ones. To achieve this goal, we have obtained a collection of Osm-r strains based on soil nitrogen-fixing bacteria without the use of genetically modified technologies, which is an innovation in sphere of soil microbiology, and, especially, in nitrogen-fixing microorganisms. These mutants were obtained on the basis of Agrobacterium sp. Y-2 and Agrobacterium sp. M-1 nitrogen-fixing strains, both spontaneously and induced. Four strains with the higher nitrogen-fixing ability, which kept their vital activity in an environment with a high concentration of salts, were selected from collection of mutants. Selected strains in the future can become the basis for creating a new, effective, environmentally friendly biofertilizer for saline soils because they are plasmidless and have the highest priority for intensive use in agriculture.

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Data sharing not applicable to this article as no datasets were generated or analyzed during the current study.

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Acknowledgements

Thanks to the Science Committee of the Republic of Armenia for financing of this research.

Funding

The study was funded by the Science Committee of the Ministry of Education and Science of the Republic of Armenia within the framework of the Armenian-Belarusian International Project AB16-52 RA MES SCN-FFIRB-2016 (2017–2019). Thanks to the funding, some chemicals were purchased and the identification of nitrogen-fixing strains was carried out.

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Authors and Affiliations

  1. Scientific and Production Center “Armbiotechnology” NAS RA (Laboratory of Strain-Producers of BAS and Biosynthesis), Gyurjyan 14, 0056, Yerevan, Armenia

    Susanna Kazar Keleshyan, Zhaneta Vladimir Karapetyan, Anna Gevorg Toplaghaltsyan, Gayane Yervand Avetisova, Lusine Hovhannes Melkonyan, Andranik Akob Vardanyan & Vahe Tariel Ghochikyan

  2. Yerevan State University (Chair of Pharmaceutical Chemistry and Pharmacognosy), Alex Manoogian 1, 0025, Yerevan, Armenia

    Gayane Yervand Avetisova & Lusine Hovhannes Melkonyan

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  1. Susanna Kazar Keleshyan
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  2. Zhaneta Vladimir Karapetyan
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  7. Vahe Tariel Ghochikyan
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Contributions

All authors contributed to the work conception and design. Conceptualization: SKK, AAV; Methodology: GYA, LHM, ZVK; Formal analysis and investigation: SKK, ZVK, AGT; Writing—original draft preparation: SKK; Writing—review and editing: SKK, VTG; Funding acquisition: SKK, ZVK, AGT, GYA; Resources: VTG; Supervision: KK, AAV. All authors read and approved the final manuscript.

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Correspondence to Susanna Kazar Keleshyan.

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Susanna Keleshyan, Zhaneta Karapetyan, Anna Toplaghaltsyan, Gayane Avetisova, Lusine Melkonyan, Andranik Vardanyan, Vahe Ghochikyan declare that they have no conflict of interest.

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Keleshyan, S.K., Karapetyan, Z.V., Toplaghaltsyan, A.G. et al. Obtaining Osmo-resistant Mutants in Nitrogen-Fixing Bacteria Isolated from Saline Soils. Curr Microbiol 79, 251 (2022). https://doi.org/10.1007/s00284-022-02948-9

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