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Comparative roles of three adhesin genes (adh1–3) in insect-pathogenic lifecycle of Beauveria bassiana

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Abstract

Adherence of conidia to insect integument is crucial for initiation of fungal infection through cuticular penetration and was previously reported to rely upon the Metarhizium-type adhesin Mad1 rather than Mad2, another adhesin crucial for conidial adherence of Metarhizium anisopliae to plant root surface. Mad1 and Mad2 have since been considered to function in fungal insect pathogenesis and plant root colonization respectively. Here, three adhesins were characterized in Beauveria bassiana, including Adh1/Mad1, Adh2/Mad2, and Adh3 known as filamentous hemagglutinin/adhesin and virulence factor in animal-pathogenic bacteria. Among those, only Adh2 was found to play a substantial role in sustaining the fungal virulence and some phenotypes associated with biological control potential. Disruption of adh2 resulted in decreased conidial adherence to insect wing cuticle, attenuated virulence via normal cuticle infection or cuticle-bypassing infection (injection), reduced blastospore production in an insect hemolymph-mimicking broth, largely reduced conidiation capacity, impaired conidial quality indicative of lowered viability, hydrophobicity, and UV resistance, but no growth defects on rich and scant media under normal or stressful culture conditions. The main phenotypic changes correlated well with repressed expression of developmental activator genes required for aerial conidiation and submerged blastospore production and of key hydrophobin genes essential for hydrophobin synthesis and assembly into rodlet bundles of conidial coat crucial for conidial adherence. In contrast, either adh1 or adh3 disruption caused insignificant changes in all phenotypes examined. These findings offer novel insight into a significance of Adh2, but a dispensability of Adh1 or Adh3, for insect-pathogenic lifecycle of B. bassiana.

Key points

• Three adhesins (Adh1–3) of Beauvera bassiana are functionally characterized.

• Adh2 plays a role in sustaining virulence and lifecycle-related cellular events.

• Either Adh1 or Adh3 is dispensable for insect-pathogenic lifecycle of B. bassiana.

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Data availability

All data generated or analyzed during this study are included in this published article (and its supplementary information files).

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Funding

Funding of this study was provided by the National Natural Science Foundation of China (Grant No. 31772218) and the Ministry of Science and Technology of the People’s Republic of China (Grant No. 2017YFD0201202).

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Authors and Affiliations

  1. MOE Laboratory of Biosystems Homeostasis & Protection, College of Life Sciences, Zhejiang University, Hangzhou, 310058, Zhejiang, China

    Qi Zhou, Lei Yu, Sheng-Hua Ying & Ming-Guang Feng

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  1. Qi Zhou
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  2. Lei Yu
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  3. Sheng-Hua Ying
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  4. Ming-Guang Feng
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Contributions

MGF and QZ conceived and designed research. QZ, LY, and SHY conducted experiments. MGF contributed new reagents or analytical tools. MGF and QZ analyzed data. MGF wrote and revised the manuscript with input from all authors. All authors read and approved the manuscript.

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Correspondence to Ming-Guang Feng.

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Zhou, Q., Yu, L., Ying, SH. et al. Comparative roles of three adhesin genes (adh1–3) in insect-pathogenic lifecycle of Beauveria bassiana. Appl Microbiol Biotechnol 105, 5491–5502 (2021). https://doi.org/10.1007/s00253-021-11420-w

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