Abstract
Glutathione peroxidase (GPX) is one of the most important antioxidant enzymes for maintaining reactive oxygen species (ROS) homeostasis. Although studies on fungi have suggested many important physiological functions of GPX, few studies have examined the role of this enzyme in Basidiomycetes, particularly its functions in fruiting body developmental processes. In the present study, GPX-silenced (GPxi) strains were obtained by using RNA interference. The GPxi strains of Hypsizygus marmoreus showed defects in mycelial growth and fruiting body development. In addition, the results indicated essential roles of GPX in controlling ROS homeostasis by regulating intracellular H2O2 levels, maintaining GSH/GSSG balance, and promoting antioxidant enzyme activity. Furthermore, lignocellulose enzyme activity levels were reduced and the mitochondrial phenotype and mitochondrial complex activity levels were changed in the H. marmoreus GPxi strains, possibly in response to impediments to mycelial growth and fruiting body development. These findings indicate that ROS homeostasis has a complex influence on growth, fruiting body development, GSH/GSSG balance, and carbon metabolism in H. marmoreus.
Key points
• ROS balance, energy metabolism, fruiting development.
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We thank **g Zhao for giving some helps in performing the experiments and thank the AJE company for providing the language editing help.
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This work was funded by the National Natural Science Foundation of China (Grant No. 31601802) and the earmarked fund of Shanghai Modern Edible Fungi-Industry Technology Research System (202009).
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****g Zhang, Hui Chen, Mingjie Chen, and Zhiyong Feng conceived and designed research. ****g Zhang, Haibo Hao, and Xuelan Wu conducted experiments. Haibo Hao and Hui Chen contributed new reagents or analytical tools. ****g Zhang and Qian Wang analyzed data. ****g Zhang wrote the manuscript. All authors read and approved the manuscript.
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Zhang, J., Hao, H., Wu, X. et al. The functions of glutathione peroxidase in ROS homeostasis and fruiting body development in Hypsizygus marmoreus. Appl Microbiol Biotechnol 104, 10555–10570 (2020). https://doi.org/10.1007/s00253-020-10981-6
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DOI: https://doi.org/10.1007/s00253-020-10981-6