Abstract
Many marine natural products hold great potential for the development of new and much needed drugs. However, the production of active metabolites by marine-derived microorganisms is usually very low, and large-scale culture has to be involved to meet the need of chemical structural modification and deep pharmacy study. In order to enhance the production of a novel cytotoxic sulfur-containing chromone oxalicumone A (OA), germinating spores of a marine-derived wild strain Penicillium oxalicum SCSGAF 0023 were mutated by microwave and ultraviolet light irradiation, which led to the obtainment of a mutant P. oxalicum SCSIO 24–2 that could produce fivefold increase in OA production (3.42 ± 0.21 mg/l) as compared to the wild strain. This is the first report that germinating spores are applied in marine-derived Penicillium sp. mutating to enhance the production of OA. Further, Plackett–Burman design and central composite design were adopted to optimize the basic medium components for increasing OA production by the mutant SCSIO 24–2 in shake flasks. The results indicated that three medium components including mannitol, maltose, and l-cysteine had significant effects on OA production, and their concentrations were optimized as 36, 27.9, and 0.99 g/l, respectively. In the optimized medium, the OA production (18.31 ± 0.27 mg/l) by mutant SCSIO 24–2 was 4.4-fold higher than that in the basic medium. These results of this work promise to improve the present production of OA and may be adopted to enhance other objective products' production by marine-derived fungi.
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Acknowledgments
Finances were supported by the National Marine Public Welfare Research Project of China (201305017), National High Technology Research and Development Program of China (863 Program, 2012AA092104), National Basic Research Program of China (2010CB833803), National Natural Science Foundation of China (41206139), National Key Technologies R&D Program (2011BAE06B04-03), and Guangdong Natural Science Foundation of China (S2011040000144).
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Zhang, XY., Bao, J., Zhong, J. et al. Enhanced production of a novel cytotoxic chromone oxalicumone A by marine-derived mutant Penicillium oxalicum SCSIO 24–2. Appl Microbiol Biotechnol 97, 9657–9663 (2013). https://doi.org/10.1007/s00253-013-5203-4
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DOI: https://doi.org/10.1007/s00253-013-5203-4