Abstract
We isolated and characterized novel insoluble phosphate (P)-solubilizing bacteria tolerant to environmental factors like high salt, low and high pHs, and low temperature. A bacterium M6 was isolated from a ginseng rhizospheric soil and confirmed to belong to Burkholderia vietnamiensis by BIOLOG system and 16S rRNA gene analysis. The optimal cultural conditions for the solubilization of P were 2.5% (w/v) glucose, 0.015% (w/v) urea, and 0.4% (w/v) MgCl2·6H2O along with initial pH 7.0 at 35°C. High-performance liquid chromatography analysis showed that B. vietnamiensis M6 produced gluconic and 2-ketogluconic acids. During the culture, the pH was reduced with increase in gluconic acid concentration and was inversely correlated with P solubilization. Insoluble P solubilization in the optimal medium was about 902 mg l−1, which was approximately 1.6-fold higher than the yield in NBRIP medium (580 mg l−1). B. vietnamiensis M6 showed resistance against different environmental stresses like 10–45°C, 1–5% (w/v) salt, and 2–11 pH range. The maximal concentration of soluble P produced by B. vietnamiensis M6 from Ca3(PO4)2, CaHPO4, and hydroxyapatite was 1,039, 2,132, and 1,754 mg l−1, respectively. However, the strain M6 produced soluble P with 20 mg l−1 from FePO4 after 2 days and 100 mg l−1 from AlPO4 after 6 days, respectively. Our results indicate that B. vietnamiensis M6 could be a potential candidate for the development of biofertilizer applicable to environmentally stressed soil.
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Park, KH., Lee, OM., Jung, HI. et al. Rapid solubilization of insoluble phosphate by a novel environmental stress-tolerant Burkholderia vietnamiensis M6 isolated from ginseng rhizospheric soil. Appl Microbiol Biotechnol 86, 947–955 (2010). https://doi.org/10.1007/s00253-009-2388-7
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DOI: https://doi.org/10.1007/s00253-009-2388-7