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Differential expression and ligand binding indicate alternative functions for zebrafish polymeric immunoglobulin receptor (pIgR) and a family of pIgR-like (PIGRL) proteins

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Abstract

The polymeric immunoglobulin (Ig) receptor (pIgR) is an integral transmembrane glycoprotein that plays an important role in the mammalian immune response by transporting soluble polymeric Igs across mucosal epithelial cells. Single pIgR genes, which are expressed in lymphoid organs including mucosal tissues, have been identified in several teleost species. A single pigr gene has been identified on zebrafish chromosome 2 along with a large multigene family consisting of 29 pigr-like (PIGRL) genes. Full-length transcripts from ten different PIGRL genes that encode secreted and putative inhibitory membrane-bound receptors have been characterized. Although PIGRL and pigr transcripts are detected in immune tissues, only PIGRL transcripts can be detected in lymphoid and myeloid cells. In contrast to pIgR which binds Igs, certain PIGRL proteins bind phospholipids. PIGRL transcript levels are increased after infection with Streptococcus iniae, suggesting a role for PIGRL genes during bacterial challenge. Transcript levels of PIGRL genes are decreased after infection with Snakehead rhabdovirus, suggesting that viral infection may suppress PIGRL function.

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Acknowledgments

We thank John Whitesides and Patti McDermott (Duke Human Vaccine Institute) for assistance with cell sorting; John Rawls (Duke University), Karen Guillemin, and Erika Mittge (University of Oregon), and Ed Noga (North Carolina State University) for bacteria; and Barb Pryor for editorial assistance. The zebrafish PIGRL gene nomenclature has been approved by the Zebrafish Nomenclature Committee. The authors are supported the National Institutes of Health (R01 AI057559 to GWL and JAY and R01 AI23337 to GWL).

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  1. Jibing Yang

    Present address: Pulmonary and Critical Care Medicine, University of Michigan, Ann Arbor, MI, USA

Authors and Affiliations

  1. Department of Molecular Biomedical Sciences and Center for Comparative Medicine and Translational Research, North Carolina State University, 1060 William Moore Drive, Raleigh, NC, 27607, USA

    Amanda N. Kortum, Ivan Rodriguez-Nunez, Jibing Yang, Poem M. Turner & Jeffrey A. Yoder

  2. Department of Molecular and Biomedical Sciences, University of Maine, Orono, ME, 04469, USA

    Juyoung Shim & Carol H. Kim

  3. Department of Immunology and Microbiology, Wayne State School of Medicine, Detroit, MI, 48201, USA

    Donna Runft & Melody N. Neely

  4. Department of Pediatrics, University of South Florida Morsani College of Medicine, 140 7th Avenue South, St. Petersburg, FL, 33701, USA

    Marci L. O’Driscoll, Robert N. Haire, John P. Cannon, Ronda T. Litman & Gary W. Litman

  5. Department of Molecular Genetics, All Children’s Hospital Johns Hopkins Medicine, 501 6th Avenue South, St. Petersburg, FL, 33701, USA

    Gary W. Litman

  6. H. Lee Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL, 33612, USA

    Gary W. Litman

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Correspondence to Jeffrey A. Yoder.

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Kortum, A.N., Rodriguez-Nunez, I., Yang, J. et al. Differential expression and ligand binding indicate alternative functions for zebrafish polymeric immunoglobulin receptor (pIgR) and a family of pIgR-like (PIGRL) proteins. Immunogenetics 66, 267–279 (2014). https://doi.org/10.1007/s00251-014-0759-4

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