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Effect of phosphate salts and varying quantities of casein and whey protein on the syrup characteristics of a sweetened condensed skimmed milk and vegetable fat blend

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Abstract

A blend of sweetened condensed skimmed milk and vegetable fat is a product that can be prepared by mixing milk constituents and/or whey in powder form with the addition of sugar and/or vegetable fat. The aim of this study was to determine the influence of varied casein and whey protein compositions, as well as the use of phosphate salts, on the stability of milk mixture syrup. Syrup production was carried out using a mixture of skimmed milk powder, demineralized whey powder, glucose, vegetable fat, sugar, and phosphate. The casein: whey protein ratios used were 80:20, 70:30, and 56:44, and the phosphates were PQTS (phosphate salt composed of sodium phosphate, and sodium citrate) and OPSS (phosphate salt composed of orthophosphate, polyphosphate, and sodium citrate). The results indicated that the PQTS salt presented the best stability of the mixture, mainly in the 56:44 mixture. In addition, it modified the pH of the mixture to a greater extent than the standard and presented particles with larger sizes at both d10 and d90 (minimum and maximum particle size and represents 10 and 90% of the volume percentage distribution, respectively). However, the OPSS salt induced a higher phosphate content in the mixture, consequently lowering the free calcium and total calcium content. The mixture produced with the 56:44 ratio exhibited a reduction in the particle size of the mixtures with phosphates when evaluating the d90. As a result, the industry will select one of these phosphates based on the qualities of the desired end product.

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References

  1. Codex alimentarius (2006) A blend of sweetened condensed skimmed milk and vegetable fat–Spectification Codex Stan 252

  2. Renhe IRT, Perrone IT, Silva PHF (2011) Leite condensado: Identidade, qualidade e tecnologia. Ed. Tempo Juiz de Fora 232p

  3. Santos MC, Oliveira JN, Silva PHF, Perrone IT, Renhe IST (2009) Avaliação de aspectos normativos do leite condensado brasileiro e no mundo. Rev Inst Laticinios Cândido Tostes 64:39–47

    Google Scholar 

  4. Conab (2008) Leite e derivados–Análise mensal. https://www.conab.gov.br>

  5. Codex alimentarius (1999). Codex Stan. http://www.fao.org/fao-who-codexalimentarius/codex-texts/list-standards/en/. Food and Agriculture Organization 1999. 212

  6. Tan R (2009) Manufacture of sweetened condensed milk and the significance of lacotse therein. In: McSweeney PLH, Fox PF (eds) Advanced dairy chemistry, Lactose, Water Salt and minor constituents. Springer Science and Business Media, New York

    Google Scholar 

  7. Smykov IT, Gnezdilova AI, Vinogradova YUV, Muzykantova AV, Lyamina AK (2019) Cooling curve in production sweetened concentrated milk supplemented with whey: Influence on the size and microstructure of lactose crystals. Food Sci Technol Int 25:451–461. https://doi.org/10.1177/1082013219830494

    Article  CAS  PubMed  Google Scholar 

  8. Silva CV (2016) Desenvolvimento de uma mistura láctea a base de soro de leite em substituição ao leite condensado para emprego na produção de sobremesas industriais. Dissertação de mestrado em Engenharia Quimica UFMG Universidade Federal de Minas Gerais 75

  9. Ozcan T, Lucey JA, Horne DS (2008) Effect of tetrasodium pyrophosphate on the physicochemical properties of yogurt gels. J Dairy Sci 91:4492–4500. https://doi.org/10.3168/jds.2008-1292

    Article  CAS  PubMed  Google Scholar 

  10. Mekmene O, Quillard S, Rouillon T, Bouler JM, Piot M, Gaucheron F (2009) Effects of pH and Ca/P molar ratio on the quantity and crystalline structure of calcium phosphates obtained from aqueous solutions. Dairy Sci Technol 89:301–316. https://doi.org/10.1051/dst/2009019

    Article  CAS  Google Scholar 

  11. Ozcan T, Horne D, Lucey JA (2011) Effect of increasing the colloidal calcium phosphate of milk on the texture and microstructure of yogurt. J Dairy Sci 94:5278–5288. https://doi.org/10.3168/jds.2010-3932

    Article  CAS  PubMed  Google Scholar 

  12. Salek RN, Vašina M, Lapčik L, Černíková M, Lorencová E, Li P, Buňka F (2019) Evaluation of various emulsifying salts addition on selected properties of processed cheese sauce with the use of mechanical vibration dam** and rheological methods. LWT Food Sci Technol 107:178–184. https://doi.org/10.1016/j.lwt.2019.03.022

    Article  CAS  Google Scholar 

  13. Saricay Y, Hettiarachchi CA, Culler MD, Harte FM (2019) Critical phosphate salt concentrations leading to altered micellar casein structures and functional intermediates. J Dairy Sci 102:6820–6829. https://doi.org/10.3168/jds.2018-15746

    Article  CAS  PubMed  Google Scholar 

  14. Costa, CHF (2016) Avaliação microestrutural do leite pasteurizado submetido ao teste do álcool visando processamento UHT. Dissertação de mestrado em Ciência e Tecnologia do Leite e Derivados UFJF Universidade Federal de Juiz de Fora 103

  15. Ho QT, Murphy KM, Drapala KP, O’Callaghan TF, Fenelon MA, O’Mahony JA, McCarthy NA (2018) Effect of pH and heat treatment on viscosity and heat coagulation properties of milk protein concentrate. Int Dairy J 85:219–224. https://doi.org/10.1016/j.idairyj.2018.05.012

    Article  CAS  Google Scholar 

  16. International Organizarion for standardization (2004). Cheese and processed cheese Determination of the total solids content. ISO standard. https://www.iso.org/standard/35249.html. 5534

  17. International Organization for Standardization (2014) Milk and milk products – Determination of nitrogen content Part 1: Kjeldahl principle and crude protein calculation. ISO standard No 8968–1. https://www.iso.org/standard/61020.html. 2014

  18. International Dairy Federation (1964) Determination of the ash content of processed cheese products. IDF Stand. 27

  19. Rios HCS, Pereira IRO, Abreu ESd (2013) Avaliação da oxidação de óleos, gorduras e azeites comestíveis em processo de fritura. Cienc Saude 6:118–126. https://doi.org/10.15448/1983-652X.2013.2.13143

    Article  Google Scholar 

  20. Pereira JPF, Paula IL, Stephani R, Perrone Í, Oliveira LFC, Carvalho AF (2019) Water versus lactose solution as a dispersion médium for particle analysis in sweetened condensed milk by laser diffraction. Quim Nova 42:928–931. https://doi.org/10.21577/0100-4042.20170406

    Article  CAS  Google Scholar 

  21. Torres EAFS, Campos NC, Duarte M, Garbelotti ML, Philippi ST (2000) Composição centesimal e valor calórico de alimentos de origem animal. Ciênc Tecnol Aliment 20:145–150. https://doi.org/10.1590/S0101-20612000000200003

    Article  Google Scholar 

  22. AOAC International (1966) Phosphorus in animal feed and pet food. AOAC. 965: 17–1966

  23. Ferreira DF (2011) Sisvar: A computer statistical analysis system. Cienc Agrotec 35:1039–1042. https://doi.org/10.1590/S1413-70542011000600001

    Article  Google Scholar 

  24. Anema SG (2016) The thermal denaturation of the total whey protein in reconstituted whole milk. Int J Dairy Technol 69:1–7. https://doi.org/10.11111/1471-0307.12356

    Article  Google Scholar 

  25. Anema SG (2014) The whey proteins in milk: thermal denaturation, physical interactions and effects on the functional properties of milk. In: Thompson A (ed) Milk Proteins. Academic Press. USA, CA

    Google Scholar 

  26. Anema SG, Lee SK, Klosterneyer H (2006) Effect of protein, non-protein-soluble components, and lactose concentrations on the irreversible thermal denaturation of β-lactoglobulin and α-lactalbumin in skim milk. J Agric Food Chem 54:7339–7348. https://doi.org/10.1021/jf061508+

    Article  CAS  PubMed  Google Scholar 

  27. Anema SG (2000) Effect of milk concentration on the irreversible thermal denaturation and disulfide aggregation of β-lactoglobulin. J Agric Food Chem 48:4168–4175. https://doi.org/10.1021/jf991173e

    Article  CAS  PubMed  Google Scholar 

  28. Anema SG, McKenna AB (1996) Reaction kinetics of thermal denaturation of whey proteins in heated reconstituted whole milk. J Agric Food Chem 44:422–428. https://doi.org/10.1021/jf950217q

    Article  CAS  Google Scholar 

  29. Kessler HG, Beyer HJ (1991) Thermal denaturation of whey proteins and its effect in dairy technology. Int J Biol Macromol 13:165–173. https://doi.org/10.1016/0141-8130(91)90043-t

    Article  CAS  PubMed  Google Scholar 

  30. Law AJR, Leaver J (2000) Effect of pH on the thermal denaturation of whey proteins in milk. J Agric Food Chem 48:672–679. https://doi.org/10.1021/jf981302b

    Article  CAS  PubMed  Google Scholar 

  31. Dumpler J, Kulozik U (2015) Heat stability of concentrated skim milk as a function of heating time and temperature on a laboratory scale – Improved methodology and kinetic relationship. Int Dairy J 49:111–117. https://doi.org/10.1016/j.idairyj.2015.05.005

    Article  CAS  Google Scholar 

  32. Lucey JA, Fox PF (1993) Importance of calcium and phosphate in cheese manufacture: A review. J Dairy Sci 76:1714–1724. https://doi.org/10.3168/jds.S0022-0302(93)77504-9

    Article  CAS  Google Scholar 

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Acknowledgments

The authors are thankful to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brasília, DF, Brazil, Financial code 001), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brasília, DF, Brazil) and Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG, Belo Horizonte, MG, Brazil)

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AFC was responsible for conceptualization, funding acquisition, and supervision of the activities. FLS, EFM, ITP, RS, and AFC designed the study. FLS, ILP, JAC, and NSC conducted laboratory analysis and validated the results. FLS and AFC were responsible for data analysis. FLS wrote the original manuscript draft. FLS, EFM, ITP, RS, and AFC reviewed and edited the manuscript.

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Correspondence to Antônio Fernandes de Carvalho.

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da Silva, F.L., Mauricio, É.F., de Paula, I.L. et al. Effect of phosphate salts and varying quantities of casein and whey protein on the syrup characteristics of a sweetened condensed skimmed milk and vegetable fat blend. Eur Food Res Technol 249, 2741–2747 (2023). https://doi.org/10.1007/s00217-023-04307-9

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