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Characterization of endogenous peptides from Dromedary and Bactrian camel milk

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Abstract

Camel milk has both nutritional value and therapeutic effects due to its bioactive components, including proteins and peptides. This study characterize endogenous peptide and potential bioactivity in both Dromedary and Bactrian camel by peptidomics techniques. In total, 622 parent protein from 8393 peptides were identified from camel milk, of which 208 proteins from Dromedary and 464 proteins from Bactrian. After filtration, 4464 endogenous peptides were quantified with 459 peptides were common in two breeds. Finally, 170 peptides were significantly different between Dromedary and Bactrian camel milk, which derived from 27 proteins, including osteopontin, lactoperoxidase up-regulated in Dromedary camel milk and butyophilin subfamily member A1, perilicin, fatty acids synthase up-regulated in Bactrian camel milk. Peptide ranker showed that 14.6% and 15.7% quantified peptides from Dromedary and Bactrian has bioactivity, which were dominated by dipeptidyl peptidase IV inhibitor (39.93%), followed by ACE inhibitor (34.85%) and anti-oxidative activity (8.69%). In sum, although Dromedary and Bactrian camel milk had significantly differences in qualitative and quantitative level of endogenous peptides, they had similarity in bioactivity including anti-diabetic, anti-hypertensive, and anti-oxidative function. The result of this study suggest that endogenous peptides may also contribute to the therapeutic benefits of camel milk.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31801463), Natural Science Foundation of Jiangsu Province (BK20180612), Innovation and Exploration Project of State Key Laboratory of Food Science and Technology (SKLF-ZZA-202104).

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Authors and Affiliations

  1. State Key Laboratory of Food Science & Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China

    Lina Zhang, Binsong Han & Peng Zhou

  2. School of Food Science and Technology, Shihezi University, Shihezi, 832000, China

    Baolong Luo & Yongqing Ni

  3. School of Agriculture and Food Science, Faculty of Science, The University of Queensland, St Lucia, 4072, Australia

    Nidhi Bansal

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  1. Lina Zhang
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Contributions

LZ: Conceptualization, formal analysis, investigation, methodology, writing—original draft, funding acquisition. BH: Data curation, methodology. BL, YN, NB: Sample collection, review & editing. PZ: Supervision, writing—review & editing.

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Correspondence to Peng Zhou.

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217_2021_3952_MOESM1_ESM.tif

Figure S1 The functional distribution of potential bioactive peptides from both Dromedary and Bactrian camel milk (A) and The differences in functional distribution of potential bioactive peptides between Dromedary and Bactrian camel milk (B) (TIF 930 KB)

217_2021_3952_MOESM2_ESM.xlsx

Table S1 Identified parent proteins of Dromedary camel milk. Table S2 Identified parent proteins of Bactrian camel milk. Table S3 Identified peptides from Dromedary camel milk. Table S4 Identified peptides from Bactrian camel milk. Table S5 Quantified peptides from Dromedary camel milk. Table S6 Quantified peptides from Bactrian camel milk. Table S7 Significantly different proteins between Dromedary and Bactrian camel milk. Table S8 Potential bioactive score of significantly different peptides using peptide ranker. Table S9 The bioactivity of those potential bioactive peptides from both Dromedary and Bactrian camel using BioPEP (XLSX 1734 KB)

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Zhang, L., Han, B., Luo, B. et al. Characterization of endogenous peptides from Dromedary and Bactrian camel milk. Eur Food Res Technol 248, 1149–1160 (2022). https://doi.org/10.1007/s00217-021-03952-2

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