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A novel method for the determination of total protein in human serum by near infrared fluorescence recovery

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Abstract

A novel fluorometric method has been developed for the determination of total protein in human serum with a new near-IR reagent as a fluorescence probe, based on the fluorescence recovery of the cyanine-CTAB system in the presence of protein. Maximum fluorescence is produced with maximum excitation and emission wavelengths at 765 and 812 nm, respectively. Under optimal conditions, the calibration graphs are linear over the ¶range 0.4–12.0 μg/mL for protein. The detection limit is 70 ng/mL, and the relative standard deviation of six replicate measurements is 1.14% for 6.0 μg/mL protein. The results are satisfactory.

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Authors and Affiliations

  1. Department of Chemistry, the Key Laboratory of Analytical Sciences of MOE, **amen University, **amen, 361005, P.R.China e-mail: jgxu@xmu.edu.cn, , , , , , CN

    Hong Zheng, Dong-Hui Li, Qiu-Ying Chen, Huang-Hao Yang, **ao-Lan Chen & **-Gou Xu

  2. Department of Chemistry, Anhui Normal University, Wuhu, 241000, P.R.China, , , , , , CN

    Chang-Qing Zhu

Authors
  1. Hong Zheng
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  2. Chang-Qing Zhu
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  3. Dong-Hui Li
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  4. Qiu-Ying Chen
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  5. Huang-Hao Yang
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  6. **ao-Lan Chen
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  7. **-Gou Xu
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Additional information

Received: 22 December 1999 / Revised: 11 April 2000 / Accepted: 19 April 2000

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Zheng, H., Zhu, CQ., Li, DH. et al. A novel method for the determination of total protein in human serum by near infrared fluorescence recovery. Fresenius J Anal Chem 368, 511–515 (2000). https://doi.org/10.1007/s002160000474

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  • DOI: https://doi.org/10.1007/s002160000474

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