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Rapid detection of three mycotoxins in animal feed materials using competitive ELISA-based origami microfluidic paper analytical device (μPAD)

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Abstract

We report the development of a competitive ELISA-based origami microfluidic paper-based analytical device (μPAD) for the detection of mycotoxins in animal feed material. The μPAD was patterned using the wax printing technique with the design of a testing pad in the middle and two absorption pads at the side. Anti-mycotoxin antibodies were effectively immobilized on chitosan–glutaraldehyde-modified sample reservoirs in the μPAD. The determination of zearalenone, deoxynivalenol, and T-2 toxin in corn flour was successfully achieved by performing competitive ELISA on the μPAD in 20 min. Colorimetric results were easily distinguished by the naked eye with a detection limit of 1 µg/mL for all three mycotoxins. The μPAD integrated with competitive ELISA holds potential for practical applications in the livestock industry for rapid, sensitive, and cost-effective detection of different mycotoxins in animal feed materials.

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Funding

This study was supported by Alberta Agriculture and Forestry, Alberta Canola Producers Commission, and Collaborative Research and Development Grant from the Natural Sciences and Engineering Research Council of Canada (ACPC103MD2018, 2018F180R, CRDPJ532306).

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Authors and Affiliations

  1. Food, Nutrition and Health Program, Faculty of Land and Food Systems, The University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

    Shaolong Feng, Marti Z. Hua & **aonan Lu

  2. Department of Food Science and Agricultural Chemistry, McGill University Macdonald Campus, 21111 Lakeshore Road, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada

    Marti Z. Hua & **aonan Lu

  3. Department of Agricultural, Food, and Nutrition Science, University of Alberta, Edmonton, AB, T6G 2R3, Canada

    M. S. Roopesh

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  1. Shaolong Feng
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  2. Marti Z. Hua
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  3. M. S. Roopesh
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  4. **aonan Lu
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Correspondence to **aonan Lu.

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Feng, S., Hua, M.Z., Roopesh, M.S. et al. Rapid detection of three mycotoxins in animal feed materials using competitive ELISA-based origami microfluidic paper analytical device (μPAD). Anal Bioanal Chem 415, 1943–1951 (2023). https://doi.org/10.1007/s00216-023-04612-y

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  • DOI: https://doi.org/10.1007/s00216-023-04612-y

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