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Using aptamers to visualize and capture cancer cells

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Abstract

Since diseased cells exist in exceedingly low concentration at the early stage of cancer, highly sensitive imaging and detection methods are required. By improving the methods for capturing and visualizing cancer cells, clinicians can diagnose metastatic relapse, stratify patients for therapeutic purposes, monitor response to drugs and therapies, and track tumor progression. Therefore, using advanced biotechnological and analytical methods combined with cell-SELEX (systematic evolution of ligands by exponential enrichment)-based aptamers, we improved the capture and visualization of diseased cells in a manner that is inexpensive, simple, sensitive, and fast. This multiplexed cancer detection platform therefore improves our control over a range of clinical exigencies, including cancer diagnosis, therapeutic modalities, and drug delivery systems.

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Acknowledgements

We thank our co-workers whose work is reported here. This work was supported by US NIH grants, China Natural Science grants and China 2009ZX10004-312. LHX thanks the support from Department of Health, Hunan Province.

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Authors and Affiliations

  1. **angya Hospital, Central South University, P.O. Box 190, Changsha, Hunan, 410008, China

    Ying Pu, Huixia Liu, Jiani Zhang & Jun Liu

  2. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China

    Weihong Tan

  3. Department of Chemistry, Center for Research at Bio/nano Interface, Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL, 32611-7200, USA

    Ying Pu, Zhi Zhu & Weihong Tan

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  1. Ying Pu
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  2. Zhi Zhu
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  5. Jun Liu
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Pu, Y., Zhu, Z., Liu, H. et al. Using aptamers to visualize and capture cancer cells. Anal Bioanal Chem 397, 3225–3233 (2010). https://doi.org/10.1007/s00216-010-3715-7

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