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Spectroscopic and voltammetric studies of Pefloxacin bound to calf thymus double-stranded DNA

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Abstract

Spectral and electrochemical studies have been carried out on the interaction of pefloxacin with calf thymus double-stranded dsDNA. The voltammetric behavior of pefloxacin was investigated at glassy carbon, carbon paste and dsDNA-modified carbon paste electrodes using cyclic voltammetry. Pefloxacin was oxidized, yielding one irreversible oxidation peak. The modification of the carbon paste surface with dsDNA allowed an accumulation process to take place for pefloxacin such that higher sensitivity was achieved compared with the bare surface. The response was characterized with respect to ionic strength, accumulation time, pefloxacin concentration, and other variables. The strip** differential pulse voltammetric response showed a linear calibration curve in the range 1.0×10−7–1.0×10−5 mol l−1 with a detection limit of 5.0×10−8 mol l−1 at the dsDNA modified electrode. The method was applied to the direct determination of pefloxacin in diluted urine samples.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Mansoura University, 34517, Dumyat, Egypt

    A. Radi

  2. National Organization for Drug Control and Research, 29, Cairo, Egypt

    M. A. EL Ries & S. Kandil

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  1. A. Radi
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  2. M. A. EL Ries
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  3. S. Kandil
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Correspondence to A. Radi.

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Radi, A., Ries, M.A.E. & Kandil, S. Spectroscopic and voltammetric studies of Pefloxacin bound to calf thymus double-stranded DNA. Anal Bioanal Chem 381, 451–455 (2005). https://doi.org/10.1007/s00216-004-2882-9

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  • DOI: https://doi.org/10.1007/s00216-004-2882-9

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