Abstract
In the present study, the individual cultures of Proteus mirabilis (P. mirabilis) and Klebsiella pneumoniae (K. pneumoniae) were treated with morphologically modified silver nanoparticles (Ag NPs) and were found to display zones of inhibition of ~ 8 mm, 16 mm, 20 mm, and 22 mm (P. mirabilis) and 6 mm, 14 mm, 20 mm, and 24 mm (K. pneumoniae) at concentrations of 25 µg/ml, 50 µg/mL, 75 µg/mL, and 100 µg/mL, respectively. In addition, turbidity tests were performed based on O. D. values, which exhibited 92% and 90% growth inhibitions at 100 µg/mL concentration for P. mirabilis and K. pneumoniae, respectively. Furthermore, the IC50 concentration of Ag NPs was established for A549 lung cancer cells and found to be at 500 µg/mL. Evidently, the morphological variation of Ag NPs treated A549 lung cancer cells was exhibited with differential morphology studied by phase-contrast microscopy. The results demonstrated that the synthesized Ag NPs was not only efficient against gram-positive bacteria but also against gram-negative bacteria and A549 cancer cells, suggesting that the potential of these biosynthesized Ag NPs is a future drug discovery source for inhibiting bacteria and cancer cells.
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Acknowledgements
G. Rajivgandhi and Franck Quero acknowledge the financial support from ANID-FONDECYT (Chile) under the Postdoctoral Fellowship No. 3220019. The authors extend their appreciation to the Researchers Supporting Project number (RSP2023R70), King Saud University, Riyadh, Saudi Arabia.
Funding
Researchers Supporting Project No. (RSP2023R70) the King Saud University, Riyadh, Saudi Arabia. G. Rajivgandhi and Franck Quero acknowledge the financial support from ANID-FONDECYT (Chile) under the Postdoctoral Fellowship No. 3220019.
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GR and FQ designed and done the nanoparticles and anti-bacterial activities experiments. GR and GC contributed in the in vitro inhibition experiments for bacteria. CKC, MM, NA, SK and MN contributed in the entire biological application process and also written the manuscript, corrected the grammatical errors, language in throughout of the manuscript. W-JL contributed entire work of the manuscript. He contributed in the structural arrangement, graphical representation and language corrections in throughout of the manuscript.
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Rajivgandhi, G., Chelliah, C.K., Ramachandran, G. et al. Morphological modification of silver nanoparticles against multi-drug resistant gram–negative bacteria and cytotoxicity effect in A549 lung cancer cells through in vitro approaches. Arch Microbiol 205, 282 (2023). https://doi.org/10.1007/s00203-023-03611-y
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DOI: https://doi.org/10.1007/s00203-023-03611-y