Abstract
Streptococcus suis is an important emerging zoonosis that causes economic losses in the pig industry and severe threats to public health. Transcriptional regulators play essential roles in bacterial adaptation to host environments. In this study, we identified a novel XRE family transcriptional regulator in S. suis CZ130302, XtrSs, involved in the bacterial fitness to hydrogen peroxide stress. Based on electrophoretic mobility shift and β-galactosidase activity assays, we found that XtrSs auto-regulated its own transcription and repressed the expression of its downstream gene psePs, a surface protein with unknown function in S. suis, by binding to a palindromic sequence from the promoter region. Furthermore, we proved that the deletion of the psePs gene attenuated bacterial antioxidant response. Phylogenetic analysis revealed that XtrSs and PsePs naturally co-existed as a combination in most S. suis genomes. Collectively, we demonstrated the binding characteristics of XtrSs in S. suis and provided a new insight that XtrSs played a critical role in modulating psePs to the hydrogen peroxide resistance of S. suis.
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Acknowledgements
We thank Shaynoor Dramsi for providing the pTCV-lac plasmid.
Funding
This work was supported by grants from the Youth Foundation of National Natural Science Foundation of China (31502085), the Fundamental Research Funds for the Central Universities (KJQN201618), the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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Communicated by Erko Stackebrandt.
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Zhang, Y., Liang, S., Pan, Z. et al. XRE family transcriptional regulator XtrSs modulates Streptococcus suis fitness under hydrogen peroxide stress. Arch Microbiol 204, 244 (2022). https://doi.org/10.1007/s00203-022-02854-5
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DOI: https://doi.org/10.1007/s00203-022-02854-5