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Microbacterium helvum sp. nov., a novel actinobacterium isolated from cow dung

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Abstract

A Gram-positive, aerobic, non-motile, non-spore-forming, short rod-shaped strain, NEAU-LLCT, was isolated from cow dung in Shangzhi City, Heilongjiang Province, Northeast China and identified by a polyphasic taxonomic study. Colonies was light yellow, round, with entire margin. Strain NEAU-LLCT was grown at 15–45 ℃ and pH 6.0–10.0. NaCl concentration ranged from 0 to 5% (W/V). The 16S rRNA gene sequence of NEAU-LLCT showed the high similarities with Microbacterium kyungheense JCM 18735T (98.5%), Microbacterium trichothecenolyticum JCM 1358T (98.3%) and Microbacterium jejuense JCM 18734T (98.2%). The whole-cell sugars were glucose, rhamnose and ribose. The menaquinones contained MK-12 and MK-13. Ornithine, glutamic acid, lysine and a small amount of alanine and glycine were the amino acids in the hydrolyzed products of the cell wall. The major fatty acids were iso-C16:0, iso-C18:0, anteiso-C15:0 and anteiso-C17:0. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The genome of NEAU-LLCT was 4,369,375 bp and G + C content is 70.28 mol%. A combination of DNA–DNA hybridization result and some phenotypic characteristics demonstrated that strain NEAU-LLCT could be distinguished from its closely related strains. Therefore, the strain NEAU-LLCT was considered to represent a novel species, which was named Microbacterium helvum sp. (Type strain NEAU-LLCT = CCTCC AA 2018026T = JCM 32661T).

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Abbreviations

ANI:

Average nucleotide identity

CCTCC:

China center for type culture collection

dDDH:

Digital DNA:DNA hybridization

DPG:

Diphosphatidylglycerol

GC–MS:

Gas chromatography–mass spectrometer

GY:

Glucose yeast extract

ISCC-NBS:

Inter-society color council-national bureau of standards

ISP:

International streptomyces project

JCM:

Japan collection of microorganisms

MEGA:

Molecular evolutionary genetics analysis

PG:

Phosphatidylglycerol

SSA:

Sodium succinate–asparagine agar

SSC:

Saline-sodium citrate

UGL:

Unidentified glycolipid

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Authors and Affiliations

  1. Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, **angfang District, Harbin, 150030, People’s Republic of China

    **ao Li, Lida Zhang, Fuyan Huang, Junwei Zhao, Han Wang, Yanjie Jiao, Lulu Qian, **ang**g Wang & Wensheng **ang

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Bei**g, People’s Republic of China

    Wensheng **ang

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  1. **ao Li
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  3. Fuyan Huang
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Contributions

XL performed the laboratory experiments, analyzed the data and drafted the manuscript. LZ contributed to the biochemical characterization. FH and HW contributed to the morphological analyzes. YJ contributed to the fatty acids determination. XY contributed to the polyphasic taxonomy. JZ participated to the discussions of each section of experiments. WX and XW designed the experiments and revised the manuscript.

Corresponding authors

Correspondence to **ang**g Wang or Wensheng **ang.

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Communicated by Erko Stackebrandt.

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Li, X., Zhang, L., Huang, F. et al. Microbacterium helvum sp. nov., a novel actinobacterium isolated from cow dung. Arch Microbiol 203, 3287–3294 (2021). https://doi.org/10.1007/s00203-021-02311-9

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