Abstract
Summary
Widespread poor vitamin D status, a health risk for bone disease, increases the need for new food sources of vitamin D. Light-exposed edible mushrooms synthesize vitamin D2. Bioavailability, safety, and efficacy of high levels of vitamin D2 from mushrooms to support bone health was established in chronically fed growing rats.
Introduction
Poor vitamin D status from reduced sun exposure is made worse by limited access to vitamin D-containing foods. Exposing white button mushrooms to ultraviolet B (UVB) light markedly increases their vitamin D2 content, creating a new food source of vitamin D. We used a growing rat model to determine safety, bioavailability, and efficacy in support of bone growth by vitamin D2 from UVB-exposed mushrooms.
Methods
We fed 150 weanling female rats one of five diets for 10 weeks, all formulated on AIN-93 G. Control diets contained no mushrooms either with or without vitamin D3. Other diets contained 2.5% and 5.0% of UVB-exposed or -unexposed mushrooms. Safety of the high levels of vitamin D2 from mushrooms was assessed by animal growth and by Von Kossa staining for soft tissue calcification. Bioavailability was determined from changes in circulating levels of 25-hydroxyvitamin D [25(OH)D] and parathyroid hormone (PTH). Efficacy in support of bone growth was determined from measures of femur bending properties, size, mineralization, and microarchitecture.
Results
Diets containing 2.5% and 5.0% light-exposed mushrooms significantly raised 25(OH)D and suppressed PTH levels compared to control-fed rats or rats fed 5.0% mushroom unexposed to light. Microarchitecture and trabecular mineralization were only modestly higher in the light-treated mushroom-fed rats compared to the controls. Von Kossa staining revealed no soft tissue calcification despite very high plasma 25(OH)D.
Conclusions
Vitamin D2 from UVB-exposed mushrooms is bioavailable, safe, and functional in supporting bone growth and mineralization in a growing rat model without evidence of toxicity.
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References
Institute of Medicine, Food and Nutrition Board (1997) Dietary reference intakes: calcium, phosphorus, magnesium, vitamin D, and fluoride. National Academy Press, Washington, DC
Whiting S, Greene T, Calvo M (2007) Vitamin D intakes in North America and Asia-Pacific Countries are not sufficient to prevent vitamin D insufficiency. J Steroid Biochem Mol Bio 103:626–630
Looker A, Pfeiffer C, Lacher D, Schleicher R, Picciano M, Yetley E (2008) Serum 25-hydroxyvitamin D status of the US population: 1988–1994 compared to 2000–2004. Am J Clin Nutr 88:1519–1527
Bailey R, Dodd K, Goldman J, Gache J, Dwyyer J, Moshfegh A, Sempos C, Picciano M (2010) Estimation of total usual calcium and vitamin D intakes in the United States. J Nutr 140:817–822
Whiting S, Langlois K, Vantanparast H, Greene-Finestone L (2011) The vitamin D status of Canadians relative to the 2011 Dietary reference intakes: an examination in children and adults with and without supplement use. Am J Clin Nutr 94:128–135
Holick M, Binkley N, Bischoff-Ferrari H, Gordon C, Hanley D, Heaney R, Murad H, Weaver C (2011) Evaluation, treatment, and prevention of vitamin D deficiency: an endocrine society clinical practice guideline. J Clin Endocrinol Metab 96:1911–1930
Calvo M, Whiting S, Barton C (2004) Vitamin D fortification in the US and Canada: current status and research needs. Am J Clin Nutr 80:1710S–17106S
Department of Agriculture, Department of Health and Human Services, Dietary guidelines for Americans 2010 [Internet]. Government Printing Office, Washington, DC; 2011 Jan 11. Available from: http://www.cnpp.usda.gov/DGAs2010-PolicyDocument.htm. Last accessed August 5, 2011
Dietary guidelines advisory committee. The report of the dietary guidelines advisory committee on the dietary guidelines for Americans, 2010. Department of Health and Human Services, Washingtion, DC, 2010. Available from: http://www.cnpp.USDA.gov/dgas2010-dgacreport.htm. Last accessed August 11, 2011
Mau J, Chen P, Yanf J (1998) Ultraviolet irradiation increased vitamin D2 content of edible mushrooms. J Agric Food Chem 46:5269–5272
Jasinghe V, Perera C (2005) Distribution of ergosterol in different tissues of mushrooms and its effect on the conversion of ergosterol to vitamin D2 by UV irradiation. Food Chem 92:541–546
Simon R, Phillips K, Horst R, Munro I (2011) Vitamin D mushrooms: comparison of the composition of button mushrooms (Agaricus bisporus) treated post harvest with UVB light or sunlight. J Agric Food Chem 59:8724–8732. Publication date: 8 July 2011
Whiting S, Calvo M (2011) Chapter 54: Lifestyle and nutritional determinants of vitamin status. In: Feldman D, Pike WJ, Adams JS (eds) Vitamin D, 3rd edn, pp 979–1007. Wiley-Blackwell
Haddad J, Matsuoka L, Hollis B, Hu Y, Wortsman J (1993) Human plasma transport of vitamin D after its endogenous synthesis. J Clin Invest 91:2552–2555
Calvo M, Whiting S, Barton C (2005) Vitamin D intake: a global perspective of current status. J Nutr 135:310–316
Hollis B (2005) Circulating 25-hydroxyvitamin D levels indicative of vitamin D sufficiency: implications for establishing a new effective dietary intake recommendation for vitamin D. J Nutr 135:317–322
Calvo M, Whiting S (2010) Chapter 18: Determinants of vitamin D intakes. In: Holick MF (ed) Vitamin D: physiology, molecular biology and clinical applications, 2nd edn, pp 361–382. Humana Press
Mattila P, Piironen V, Uusi-Rauva E, Koivistoinen P (1994) Vitamin D contents in edible mushrooms. J Agric Food Chem 42:2449–2453
Roberts J, Teichert A, McHugh T (2008) Vitamin D2 formation from post-harvest UV-B treatment of mushrooms (Agaricus bisporus) and retention during storage. J Agric Food Chem 56:4541–4544
Koyyalamudi S, Jeong S, Song C, Cho K, Pang G (2009) Vitamin D2 formation and bioavailability from Agaricus bisporus button mushroom treated with ultraviolet irradiation. J Agric Food Chem 57:3351–3355
Phillips K, Ruggio D, Horst R, Minor B, Simon R, Feeney M, Byrdwell W, Haytowitz D (2011) Vitamin D and sterol composition of ten types of mushrooms from retail suppliers in the United States. J Agric Food Chem 59:7841–7853
Babu U, Balan K, Amankwa-Sakyi M, Garthoff L, Calvo M (2011) Assessment of immuno-modulatory effects of vitamin D2-enriched mushroom consumption on LPS response using splenocyte microarray of genes regulating inflammatory cytokines, chemokines and their receptors. Mol Nutr Food Res, submitted for publication
Garthoff L, Sobotka T (2001) From farm to table to brain: foodborne pathogen infection and the potential role of the neuro-immune-endocrine system in neuro sequelae. Nutr Neurosci 4:333–374
Fleet J, Gliniak C, Xue Y, Smith K, McCreedy R, Adedokun S (2008) Serum metabolite profiles and target tissue gene expression define the effect of cholecalciferol intake on calcium metabolism in rats and mice. J Nutr 138:1114–1120
Mattila P, Lampi A-M, Ronkainen R, Tovio J, Piironen V (2002) Sterol and vitamin D2 contents in some wild and cultivated mushrooms. Food Chem 76:293–298
Jasinghe V, Perera C (2006) Ultraviolet irradiation: the generator of vitamin D2 in edible mushrooms. Food Chem 95:638–643
Müller R, Koller B, Hildebrand T, Laib A, Gianolini S, Rüegsegger P (1996) Resolution dependency of microstructural properties of cancellous bone based on three-dimensional mu-tomography. Technol Health Care 4:113–119
Hildebrand T, Rüegsegger P (1997) Quantification of bone microarchitecture with the structure model index. Comput Methods Biomech Biomed Eng 1:15–23
Hildebrand T, Laib A, Müller R, Dequecker J, Rüegsegger P (1999) Direct three-dimensional morphometric analysis of human cancellous bone microstructural data from spine, femur, iliac crest and calcaneus. J Bone Miner Res 14:1167–1174
Bills C, Eisenberg H, Pallante S (1974) Complexes of organic acids with calcium phosphate: the Von Kossa stain as a clue to the composition of bone mineral. Johns Hopkins Med J 128:194–207
Institute of Medicine, Food and Nutrition Board (2011) Dietary reference intakes for calcium and vitamin D; National Academy of Science (NAS). The National Academy Press, Washington, DC
Armas L, Hollis B, Heaney R (2004) Vitamin D2 is much less effective than vitamin D3 in humans. J Clin Endocrinol Metab 89:5387–5391
Horst R, Napoli J, Littledike T (1982) Discrimination in the metabolism of orally dosed ergocalciferol and cholecalciferol by the pig, rat and chick. Biochem J 204:185–189
Hunt R, Garcia F, Walsh R (1972) A comparison of the toxicity of ergocalciferol and cholecalciferol in rhesus monkeys. J Nutr 102:975–986
Stephenson D, Peiris A (2009) The lack of vitamin toxicity and megadose of daily ergocalciferol (D2) therapy: a case report and literature review. Southern Med J 102:765–768
Binkley N, Gemar D, Engelke J, Gangnon R, Ramamurthy R, Krueger D, Drezner M (2011) Evaluation of ergocalciferol and cholecalciferol dosing, 1,6000 IU daily or 50,000 IU monthly in older adults. J Clin Endocrinol Metab 96:981–988
Ouitila T, Mattila P, Piironen V, Lambert-Allardt C (1999) Bioavailability of vitamin D from wild edible mushrooms (Cantharellus tubaeformis) as measured with a human bioassay. Am J Clin Nutr 69:95–98
Ozzard A, Hear G, Morrison G, Hoskin M (2009) Vitamin D deficiency treated by consuming UVB-irradiated mushrooms. Br J Gen Pract 58:644–645
Urbain P, Singler F, Ihorst G, Biesalski, Bertz H (2011) Bioavailability of vitamin D2 from UV-B-irradiated button mushrooms in healthy adults deficient in serum 25-hydroxyvitamin D: a randomized controlled trial. Euro J Clin Nutr 65:965–971
Tjellesen L, Hummer L, Christiansen C, Rodbro P (1986) Serum concentration of vitamin D metabolites during treatment with vitamin D2 and D3 in normal premenopausal women. Bone Miner 1:407–413
Lee A, Anderson P, Sawyer R, Moore A, Forwood M, Steck R, Morris H, O’Loughlin P (2010) Discordant effects of vitamin D deficiency in trabecular and cortical bone architecture and strength in growing rodents. J Steroid Biochem Mol Biol 121:284–287
Biancuzzo R, Young A, Bilbuld D, Cai M, Winter M, Klein E, Ameri A, Salameh W, Chen T, Holick M (2010) Fortification of orange juice with vitamin D2 or vitamin D3 is as effective as an oral supplement in maintaining vitamin D status in adults. Am J Clin Nutr 91:1621–1626
Monsivais P, Aggarwal A, Drewnowski A (2011) Following federal guidelines to increase nutrient consumption may lead to higher food costs for consumers. Heal Aff 30:1–7
Khadilkar A, Sayyad M, Sanwalka N, Bhandari D, Naik S, Khadilkar V, Mughal M (2010) Vitamin D supplementation and bone mass accrual in underprivileged adolescent Indian girls. Asia Pac J Clin Nutr 19:465–472
Boas S, Hageman J, Ho L, Liveris M (2009) Very high-dose ergocalciferol is effective for correcting vitamin D deficiency in children and young adults with cystic fibrosis. J Cyst Fibros 8:270–272
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The Mushroom Council, and the US FDA Centers for Food Safety and Applied Nutrition and Devices and Radiological Health.
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The findings and conclusions presented in this manuscript are those of the authors and do not necessarily represent the views or opinions of the US FDA.
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Calvo, M.S., Babu, U.S., Garthoff, L.H. et al. Vitamin D2 from light-exposed edible mushrooms is safe, bioavailable and effectively supports bone growth in rats. Osteoporos Int 24, 197–207 (2013). https://doi.org/10.1007/s00198-012-1934-9
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DOI: https://doi.org/10.1007/s00198-012-1934-9