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Histologic evaluation of human cadaveric fascia lata in a rabbit vagina model

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Abstract

The purpose of this study was to evaluate the histologic response of human cadaveric fascia lata after vaginal implantation. Freeze-dried, gamma-irradiated cadaveric fascia lata from three lots was implanted between the rectovaginal membrane and vaginal epithelium in New Zealand white rabbits. Rabbits were killed at 2, 4, 8, and 12 weeks after implantation. At necropsy, gross findings were described and specimens for routine cultures were taken. Histologic evaluation determined graft integrity, neovascularization, inflammatory response, and host tissue incorporation. Nine rabbits were available for histologic analysis and 14 for gross and microbiologic analysis. Vaginal erosions occurred with three grafts. The remainder were adherent to the surrounding tissues. Erosion was associated with bacterial colonization of the graft. Autolysis of one graft occurred at 4 weeks. Over time, the inflammatory response decreased and neovascularization increased; by 12 weeks, the graft collagen was replaced by host collagen. Cadaveric fascia lata serves as scaffolding for host tissue incorporation with replacement by host collagen.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Mayo Clinic, Scottsdale, AZ, USA

    Andrew J. Walter, Abraham N. Morse & Jeffrey L. Cornella

  2. Department of Laboratory Medicine and Pathology, Mayo Clinic, Scottsdale, AZ, USA

    Kevin O. Leslie

  3. Division of Biostatistics, Mayo Clinic, Scottsdale, AZ, USA

    Joseph G. Hentz

  4. Department of Obstetrics and Gynecology, 1650 Response Road, Sacramento, CA, 95815, USA

    Andrew J. Walter

Authors
  1. Andrew J. Walter
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  2. Abraham N. Morse
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  3. Kevin O. Leslie
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  4. Joseph G. Hentz
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  5. Jeffrey L. Cornella
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Correspondence to Andrew J. Walter.

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Walter, A.J., Morse, A.N., Leslie, K.O. et al. Histologic evaluation of human cadaveric fascia lata in a rabbit vagina model. Int Urogynecol J 17, 136–142 (2006). https://doi.org/10.1007/s00192-005-1321-6

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  • DOI: https://doi.org/10.1007/s00192-005-1321-6

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