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Impact of Intensive Farming on Soil Heavy Metal Accumulation and Biomarkers Responses of Earthworms Eisenia andrei

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Abstract

In this work, we propose to evaluate the effect of agriculture intensification under greenhouses on the biochemical and transcriptomic responses of the earthworms Eisenia andrei. This work was conducted on two sites in Téboulba and Sahline (Monastir governorate) and a control site in an experimental plot that is undergoing organic farming. For this purpose, the earthworms Eisenia andrei were exposed to the soils during 7 and 14 days. The physicochemical properties of the soils were analyzed. The biochemical biomarkers of metallothioneins (MTs) and malondialdehyde (MDA) accumulations were also assessed. Moreover, the gene expression level of the MTs was analyzed. The results of our study revealed a significant trace element accumulation accompanied by a high level of MDA and MT proteins. Moreover, a significant expression of the MT gene was observed in earthworms exposed to the soils from Sahline and Téboulba. Hence, this work reveals that intensive agriculture can affect the biological responses of earthworms and consequently, the soil’s biofertility.

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Authors and Affiliations

  1. Regional Research Centre in Horticulture and Organic Agriculture, Chott-Mariem, 4042, Sousse, Tunisia

    Sabrine Hattab

  2. Laboratory of Biochemistry and Environmental Toxicology, Higher Institute of Agronomy, 4042, Chott-Meriem, Tunisia

    Iteb Boughattas, Marouane Mkhinini & Mohamed Banni

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  1. Sabrine Hattab
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  2. Iteb Boughattas
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  3. Marouane Mkhinini
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  4. Mohamed Banni
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Correspondence to Iteb Boughattas.

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Hattab, S., Boughattas, I., Mkhinini, M. et al. Impact of Intensive Farming on Soil Heavy Metal Accumulation and Biomarkers Responses of Earthworms Eisenia andrei. Bull Environ Contam Toxicol 105, 559–564 (2020). https://doi.org/10.1007/s00128-020-03000-x

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