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Elucidating Microbial Pathways of Mercury Methylation During Litter Decomposition

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Abstract

Tree foliage sequesters gaseous elemental mercury (Hg) through stomatal uptake, when the foliage senesces and falls into the water, Hg from leaf litter can be released into the water and/or microbially methylated into a highly toxic form, methylmercury. The dominant groups of microbial communities that can methylate Hg during litter decomposition are, however, less certain. We conducted a microbial inhibition experiment to identify the primary methylators of leaf litter Hg during 28-day decomposition of two litter species of contrasting quality (pine and maple). We demonstrate that sulfate-reducing bacteria are the dominant microbial groups for Hg methylation during anoxic litter decomposition, and our study also indicates that methanogens may have a minor role in mediating Hg methylation during litter decomposition. Thus, aquatic environment with extensive litter accumulation and decomposition (e.g., wetlands, ponds, and river pools) can be hotspots of Hg methylation through sulfate-reduction and, to a lesser extent, methanogenesis.

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References

  • Allan JD, Castillo MM (2007) Stream ecology: structure and function of running waters, Second edn. Springer, Netherlands

    Book  Google Scholar 

  • Balogh SJ, Huang Y, Offerman HJ, Meyer ML, Johnson DK (2002) Episodes of elevated methylmercury concentrations in prairie streams. Environ Sci Technol 36:1665–1670

    Article  CAS  Google Scholar 

  • Bloom N (1989) Determination of picogram levels of methylmercury by aqueous phase ethylation, followed by cryogenic gas chromatography with cold vapour atomic fluorescence detection. Can J Fish Aquat Sci 46:1131–1140

    Article  CAS  Google Scholar 

  • Blum PW, Hershey AE, Tsui MTK, Hammerschmidt CR, Agather AM (2018) Methylmercury and methane production potentials in North Carolina Piedmont stream sediments. Biogeochemistry 137:181–195

    Article  CAS  Google Scholar 

  • Ericksen JA, Gustin MS, Schorran DE, Johnson DW, Lindberg SE, Coleman JS (2003) Accumulation of atmospheric mercury in forest foliage. Atmos Environ 37:1613–1622

    Article  CAS  Google Scholar 

  • Fitzgerald WF, Engstrom DR, Mason RP, Nater EA (1998) The case for atmospheric mercury contamination in remote areas. Environ Sci Technol 32:1–7

    Article  CAS  Google Scholar 

  • Gilmour CC et al (2013) Mercury methylation by novel microorganisms from new environments. Environ Sci Technol 47:11810–11820

    Article  CAS  Google Scholar 

  • Gilmour CC, Henry EA, Mitchell R (1992) Sulfate stimulation of mercury methylation in freshwater sediments. Environ Sci Technol 26:2281–2287

    Article  CAS  Google Scholar 

  • Grigal DF (2002) Inputs and outputs of mercury from terrestrial watersheds: a review. Environ Rev 10:1–39

    Article  CAS  Google Scholar 

  • Hamelin S, Amyot M, Barkay T, Wang Y, Planas D (2011) Methanogens: principal methylators of mercury in lake periphyton. Environ Sci Technol 45:7693–7700

    Article  CAS  Google Scholar 

  • Horvat M, Liang L, Bloom NS (1993) Comparison of distillation with other current isolation methods for the determination of methyl mercury compounds in low level environmental samples. Part II. Water. Anal Chim Acta 282:153–168

    Article  CAS  Google Scholar 

  • Jeremiason JD et al (2006) Sulfate addition increases methylmercury production in an experimental wetland. Environ Sci Technol 40:3800–3806

    Article  CAS  Google Scholar 

  • Jiskra M et al (2018) A vegetation control on seasonal variations in global atmospheric mercury concentrations. Nat Geosci 11:244–250

    Article  CAS  Google Scholar 

  • Kerin EJ, Gilmour CC, Roden E, Suzuki MT, Coates JD, Mason RP (2006) Mercury methylation by dissimilatory iron-reducing bacteria. Appl Environ Microbiol 72:7919–7921

    Article  CAS  Google Scholar 

  • Ku P, Tsui MTK, Nie X, Chen H, Hoang TC, Blum JD, Dahlgren RA, Chow AT (2018) Origin, reactivity, and bioavailability of mercury in wildfire ash. Environ Sci Technol 52:14149–14157

    Article  CAS  Google Scholar 

  • Liang L, Horvat M, Bloom NS (1994) An improved speciation method for mercury by GC/CVAFS 165 after aqueous phase ethylation and room temperature precollection. Talanta 41:371–379

    Article  CAS  Google Scholar 

  • Marvin-DiPasquale M, Agee J, McGowan C, Oremland RS, Thomas M, Krabbenhoft D, Gilmour CC (2000) Methyl-mercury degradation pathways: a comparison among three mercury-impacted ecosystems. Environ Sci Technol 34:4908–4916

    Article  CAS  Google Scholar 

  • Obrist D et al (2011) Mercury distribution across 14 U.S. Forests. Part I: spatial patterns of concentrations in biomass, litter, and soils. Environ Sci Technol 45:3974–3981

    Article  CAS  Google Scholar 

  • Oremland RS, Culbertson CW, Winfrey MR (1991) Methylmercury decomposition in sediments and bacterial cultures: involvement of methanogens and sulfate reducers in oxidative demethylation. Appl Environ Microbiol 57:130–137

    CAS  Google Scholar 

  • Parker JL, Bloom NS (2005) Preservation and storage techniques for low-level aqueous mercury speciation. Sci Total Environ 337:253–263

    Article  CAS  Google Scholar 

  • Parks JM et al (2013) The genetic basis for bacterial mercury methylation. Science 339:1332–1335

    Article  CAS  Google Scholar 

  • Podar M et al (2015) Global prevalence and distribution of genes and microorganisms involved in mercury methylation. Sci Adv 1:e1500675

    Article  Google Scholar 

  • Pokharel AK, Obrist D (2011) Fate of mercury in tree litter during decomposition. Biogeosciences 8:2507–2521

    Article  CAS  Google Scholar 

  • Scheuhammer AM, Meyer MW, Sandheinrich MB, Murray MW (2007) Effects of environmental methylmercury on the health of wild birds, mammals, and fish. Ambio 36:12–18

    Article  CAS  Google Scholar 

  • Seller P, Kelly CA, Rudd JWM, MacHutchon AR (1996) Photodegradation of methylmercury in lakes. Nature 380:694–697

    Article  Google Scholar 

  • Tsui MTK, Finlay JC, Nater EA (2008) Effects of stream water chemistry and tree species on release and methylation of mercury during litter decomposition. Environ Sci Technol 42:8692–8697

    Article  CAS  Google Scholar 

  • Tsui MTK, Finlay JC, Nater EA (2009) Mercury bioaccumulation in a stream network. Environ Sci Technol 43:7016–7022

    Article  CAS  Google Scholar 

  • Tsui MTK, Wang WX (2004) Uptake and elimination routes of inorganic mercury and methylmercury in Daphnia magna. Environ Sci Technol 38:808–816

    Article  CAS  Google Scholar 

  • UNEP (2013) Global Mercury Assessment 2013: sources, emissions, releases and environmental transport. United Nations Environment Programme, Chemicals Branch, Geneva

    Google Scholar 

  • USEPA (2002) Method 1631, revision E: mercury in water by oxidation, purge and trap, and cold vapor atomic fluorescence spectrometry. office of water. U.S. Environmental Protection Agency, Washington, DC

    Google Scholar 

  • Woerndle GE, Tsui MTK, Sebestyen SD, Blum JD, Nie X, Kolka RK (2018) New insights on ecosystem mercury cycling revealed by stable isotopes of mercury in water flowing from a headwater peatland catchment. Environ Sci Technol 42:1854–1861

    Article  CAS  Google Scholar 

  • Yu RQ, Reinfelder JR, Hines ME, Barkay T (2013) Mercury methylation by the methanogen Methanospirillum hungatei. Appl Environ Microbiol 79:6325–6330

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported by National Science Foundation awards (DEB-1354811 and EAR-1711642) and funding from Department of Biology, the University of North Carolina at Greensboro. We appreciated the constructive comments from two anonymous reviewers on the draft of the manuscript.

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  1. Department of Biology, University of North Carolina at Greensboro, 321 McIver St, Room 312 Eberhart Building, Greensboro, NC, 27402, USA

    Elaine Chow & Martin Tsz-Ki Tsui

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  1. Elaine Chow
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  2. Martin Tsz-Ki Tsui
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Correspondence to Martin Tsz-Ki Tsui.

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Chow, E., Tsui, M.TK. Elucidating Microbial Pathways of Mercury Methylation During Litter Decomposition. Bull Environ Contam Toxicol 103, 617–622 (2019). https://doi.org/10.1007/s00128-019-02700-3

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  • DOI: https://doi.org/10.1007/s00128-019-02700-3

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