Abstract
Fourteen microsatellites containing GA-repeats were isolated and characterized in cassava (Manihot esculenta Crantz, Euphorbiaceae). Microsatellite heterozygosity (h) was estimated in 48 accessions using (32P)-end-labeled primers and in more than 500 accessions using fluorescence-based genoty**. Heterozygosity values ranged from 0.00 to 0.88 and the number of alleles detected varied from 1 to 15. The reproducibility of allele sizing was also assessed using fluorescence-based genoty**. The average inter-gel size difference was 1.03 nucleotides. Chi-square tests (χ2) were performed to analyse segregation distortion and the linkage between alleles segregating from either or both parents in an F1 map** population. Most microsatellite loci segregated in the expected 1 : 1, 1 : 2 : 1 or 1 : 1 : 1 : 1 ratio. Linkage was detected between loci segregating from either parent, and segregation distortion from the male parent was detected for locus GA-131. Approximately 80% of the microsatellites detected one or two alleles per accession, suggesting a low degree of microsatellite locus duplication, an unexpected finding for a putative allopolyploid, highly heterozygous species. The high h values of most microsatellites, their amplification in other Manihot taxa and their suitability for high-throughput, fluorescence-based genoty**, make microsatellites the marker of choice for germplasm characterization and saturation of the cassava map.
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Received: 4 September 1997 / Accepted 16 March 1998
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Chavarriaga-Aguirre, P., Maya, M., Bonierbale, M. et al. Microsatellites in Cassava (Manihot esculenta Crantz): discovery, inheritance and variability. Theor Appl Genet 97, 493–501 (1998). https://doi.org/10.1007/s001220050922
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DOI: https://doi.org/10.1007/s001220050922