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Identification of CaPCR1, an OFP gene likely involved in pointed versus concave fruit tip regulation in pepper (Capsicum annuum L.) using recombinant inbred lines

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Abstract

Key message

CaPCR1 (Capana12g002165) was a candidate gene regulating fruit concave/pointed tip shape in pepper.

Abstract

The concave shape of the fruit tip in pepper plants is highly susceptible to drought and low temperature stresses, resulting in the appearance of a pointed tip fruit, which affects its commercial value. However, few studies on the process of fruit tip development and regulatory genes in pepper have been reported. Herein, the developmental process of the ovary before anthesis, especially changes in the shape of the ovary tip, was studied in detail. The results showed that the final fruit tip shape was consistent with the ovary tip shape before anthesis, and a concave tip shape gradually developed. F4 recombinant inbred lines (RILs) were constructed to map the genes regulating fruit tip shape through hybridization of the LRS and SBS pepper inbred lines. CaPCR1 (Capana12g002165), an OFP (OVATE Family Protein) family gene, was located in the candidate region on chr12. Three SNPs were found in the protein coding sequence of CaPCR1 between SBS and LRS, but only one SNP led to amino acid variation. Sequence variations, including base replacements, deletions and insertions, were also detected in the gene promoter region. The relative expression level of the CaPCR1 gene was significantly greater in the concave tip ovary than in the pointed tip ovary. qRT‒PCR analysis revealed that the CaPCR1 gene was expressed mainly in the gynoecium, placenta and green fruit pericarp, which was consistent with its function in ovary and fruit development. Taken together, these results suggested that CaPCR1 is a candidate gene involved in fruit tip shape determination in pepper.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (2018YFD1000800) and National Natural Science Foundation of China (32172600). National Key Research and Development Program of China, 2018YFD1000800, Feng Li, National Natural Science Foundation of China, 32172600, Yingtian Deng

Funding

This study was supported by the National Key Research and Development Program of China (2018YFD1000800) and National Natural Science Foundation of China (32172600).

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Authors and Affiliations

  1. National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, Hubei, China

    Tingting Liu, Yi** Dong, Shenting Gao, Yingjia Zhou, Dan Liu, Jubin Wang, Zhenya Liu, Yingtian Deng & Feng Li

  2. Traditional Chinese Medicine College, Bozhou University, Bozhou, 236800, Anhui, China

    Tingting Liu

  3. Hubei Hongshan Laboratory, Wuhan, 430070, Hubei, China

    Feng Li

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  1. Tingting Liu
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  2. Yi** Dong
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  3. Shenting Gao
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  5. Dan Liu
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Contributions

F. L. and Y. D. conceived and designed the research; T. L. performed the research and wrote the first paper; Y. D, D. L. and S.G. conducted the experiments; Y. Z, J. W. and Z. L. analyzed the data; Y. D. participated in its design. All authors read and approved the manuscript.

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Correspondence to Yingtian Deng or Feng Li.

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Communicated by Esther van der Knaap.

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Liu, T., Dong, Y., Gao, S. et al. Identification of CaPCR1, an OFP gene likely involved in pointed versus concave fruit tip regulation in pepper (Capsicum annuum L.) using recombinant inbred lines. Theor Appl Genet 137, 161 (2024). https://doi.org/10.1007/s00122-024-04675-0

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