Abstract
Onion exhibits wide genetic and environmental variation in bioactive organosulfur compounds that impart pungency and health benefits. A PCR-based molecular marker map that included candidate genes for sulfur assimilation was used to identify genomic regions affecting pungency in the cross 'W202A' × 'Texas Grano 438'. Linkage map** revealed that genes encoding plastidic ferredoxin-sulfite reductase (SiR) and plastidic ATP sulfurylase (ATPS) are closely linked (1–2 cM) on chromosome 3. Inbred F3 families derived from the F2 population used to construct the genetic map were grown in replicated trials in two environments and bulb pungency was evaluated as pyruvic acid or lachrymatory factor. Broad-sense heritability of pungency was estimated to be 0.78–0.80. QTL analysis revealed significant associations of both pungency and bulb soluble solids content with marker intervals on chromosomes 3 and 5, which have previously been reported to condition pleiotropic effects on bulb carbohydrate composition. Highly significant associations (LOD 3.7–8.7) were observed between ATPS and SiR Loci and bulb pungency but not with bulb solids content. This association was confirmed in two larger, independently derived F2 families from the same cross. Single-locus models suggested that the partially dominant locus associated with these candidate genes controls 30–50% of genetic variation in pungency in these pedigrees. These markers may provide a practical means to select for lower pungency without correlated selection for lowered solids.
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Abbreviations
- EPA:
-
Enzymatically produced pyruvic acid
- SSC:
-
Soluble solids content
- SSCP:
-
Single-stranded conformation polymorphism
- HDX:
-
Heteroduplex
- LF:
-
Onion lachrymatory factor
- CAPS:
-
Cleaved amplified polymorphic sequence
- ACSO:
-
Alk(en)yl cysteine sulfoxide
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Acknowledgments
We acknowledge significant contributions of the following: Bodhi Bettjeman, Paul McCartney and Winston Fahey for technical support; David and Judy MacMillan and Enza Zaden NZ Ltd for field trial support. This work was funded by the New Zealand Foundation for Research, Science and Technology.
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Communicated by C. F. Quiros.
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McCallum, J., Pither-Joyce, M., Shaw, M. et al. Genetic map** of sulfur assimilation genes reveals a QTL for onion bulb pungency. Theor Appl Genet 114, 815–822 (2007). https://doi.org/10.1007/s00122-006-0479-8
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DOI: https://doi.org/10.1007/s00122-006-0479-8