Abstract
In this work, a seed-set-based screening was performed on 70 lines ofArabidopsis thaliana after activation tagging mutagenesis to identify mutations in reproductive mechanisms. Five mutants showed significantly lower seed set than the wild type and confirmed the phenotype in the progeny. This phenotype was linked with the marker genebar carried by T-DNA conferring glufosinate resistance. Genetic analysis revealed that the mutation inheritance was sporophytic in 3 mutants and gametophytic in 2 mutants. In addition, 2 mutants had an extra T-DNA copy. Thus activation tagging can be an effective strategy to identify new mutations affecting sporogenesis or gametogenesis.
References
Akama K, Shiraishi H, Ohta S, Nakamura K, Okada K, Shimura Y, 1992. Efficient transformation ofArabidopsis thaliana: comparison of the efficiencies with various organs, plant ecotypesand Agrobacterium strains. Plant Cell Rep 12: 7–11.
Consiglio F, Carputo D, Monti L, Conicella C, 2004. Exploitation of genes affecting meiotic non-reduction and nuclear restitution:Arabidopsis as a model? Sex Plant Reprod 17: 97–105.
Drews GN, Lee D, Christensen GA, 1998. Genetic analysis of female gametophyte development and function. Plant Cell 10: 5–17.
Howden R, Park SK, Moore JM, Orme J, Grossniklaus U, Twell D, 1998. Selection of T-DNA tagged male and female gametophytic mutants by segregation distortion inArabidopsis. Genetics 149: 621–631.
Ito T, Meyerowitz EM, 2000. Overexpression of a gene encoding a cytochrome P450, CYP78A9, induces large and seedless fruit inArabidopsis. Plant Cell 12: 1541–1550.
Yadegari R, Drews GN, 2004. Female gametophyte development. Plant Cell 16: 133–141.
Weigel D, Ahn JH, Blazquez MA, Borevitz JO, Christensen SK, Fankhauser C, et al. 2000. Activation tagging inArabidopsis. Plant Physiol 122: 1003–1013.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Perrella, G., Cremona, G., Consiglio, F. et al. Screening for mutations affecting sexual reproduction after activation tagging inArabidopsis thaliana . J Appl Genet 47, 109–111 (2006). https://doi.org/10.1007/BF03194608
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03194608