Abstract
The production of antitumor human monoclonal antibodies (MoAbs) by human-human or mouse-human hybridoma technology was studied. UC 729-6, a thioguanine-resistant, HAT-sensitive human lymhoblastoid B cell line or NS-1, a mouse myeloma cell line, were fused with lymphocytes isolated from regional lymph nodes of cancer patients, resulting in 130 Ig-secreting human-human hybrids and 21 human Ig-secreting mouse-human hybrids. The supernatants of 88 hybrids were screened against a panel of cancer cell lines using a microspot smear method developed in this laboratory.
The results showed that the supernatants of 37 human-human hybrids and two mouse-human hybrids reacted against a number of cancer cell lines. However, after subcloning for three times, only one human-human hybridoma (MUBL-6) and one mouse-human hybridoma (HMG-1) secreted human MoAbs in continuous culture for 9–12 months.
In an immunohistological study by PAP, MUBL-6, a human-human hybridoma derived from the lymph nodes of breast cancer patients, stained positively with malignant cells of breast cancer simplex but not with stroma cells of the same tissue section. HMG-1, a mouse-human hybridoma derived from lymphocytes of a patient with gastric adenocarcinoma secreted IgM and reacted with malignant adenoepithelial cells of gastric cancer but not with normal gastric adenoepithelial or stroma cells. There was also some complement-dependent cytotoxicity against gastric cancer cells MGC-823.
Ascites was obtained by injecting antibody-producing human-human or mouse-human hybrids into BALB/c nude mice pretreated with antilymphocyte serum and pristane. The human MoAb obtained from ascitic fluid was 100–1000 times greater than that of culture supernants.
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Liu, H., Xu, ZL., Wang, Y. et al. Production of antitumor human monoclonal antibodies by human-human or mouse-human hybridoma. Chin J Cancer Res 1, 20–28 (1988). https://doi.org/10.1007/BF02997636
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DOI: https://doi.org/10.1007/BF02997636