Summary
The cell kinetic perturbations following irradiation (20 Gy) were studied by combining the metaphase arrest method using vincristine with histological indices of cell death. The metaphase arrest method yielded remarkably constant values of rate of entry into mitosis (r M ) of around 24 new cells/1,000 cells/hour during a 7 day period in which there was no tumour growth. The time course of cell death as indicated by changes in the pyknotic index during this period may reflect the processes of reoxygenation and repopulation known to influence the results of fractionated radiotherapy.
Similar content being viewed by others
References
Brown MJ (1970) The effect of acute x-irradiation on the cell proliferation kinetics of induced carcinomas and their normal counterpart. Radiat. Research43:627–653
Chalkley HW (1943) Method for quantitative morphologic analysis of tissues. J Nat Cancer Inst4: 47–53
Denekamp J, Thomlinson RH (1971) The cell proliferation kinetics of four experimental tumours after acute x-irradiation. Cancer Res31:1279–1284
Fowler JF, Denekamp J, Sheldon PW, Smith AM, Begg AC, Harris SR, Page AL (1974) Optimum fractionation in x-ray treatment of C3H mouse mammary tumours. Br J Radiol47:781–789
Hermens AF, Barendsen GW (1969) Changes of cell proliferation characteristics in a rat rhabdomyosarcoma before and after x-irradiation. Eur J Cancer5:173–189
Howes AE(1969) An estimation of the changes in the proportions and absolute numbers of hypoxic cells in C3H mouse mammary tumours. Br J Radiol42:441–447
Maurer HR (1981) Potential pitfalls of3H thymidine techniques to measure cell proliferation. Cell Tissue Kinet14:111–120
Nias AHW, Chamberlain SM, Abdelaal AS (1980) The serially transplanted C3H mouse mammary tumour as a clinically relevant model system. Brit J Cancer (Suppl IV)41:157
Rowley R, Hopkins HA, Betsill WL, Ritenour ER, Looney WB (1980) Response and recovery kinetics of a solid tumour after irradiation. Br J Cancer42:586–595
Szczepanski LV, Trott KR (1975) Post irradiation proliferation kinetics of a serially transplanted murine adenocarcinoma. Br J Radiol45:200–208
Trott KR (1980) Can tumour response be assessed from a biopsy? Br J Cancer (Suppl IV)41:163–170
Van Peperzeel HA (1972) Effects of single doses of radiation on lung metastases in man and experimental animals. Eur J Cancer8:665–676
Wright NA, Appleton DR (1980) The metaphase arrest technique: a critical review. Cell Tissue Kinet13:643–663
Wright NA, Al-Nafussi A, Britton N (1980) Growth control in the small intestine after death of proliferating cells induced by cytosine Arabinoside. In: Appleton DR, Sunter JP, Watson AJ (eds) Cell proliferation in the gastrointestinal tract. Pitman Medical Ltd, Turnbridge Wells, Kent, pp 102–108
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jones, B., Camplejohn, R.S. Cell kinetic response of an experimental tumour to irradiation. Virchows Archiv B Cell Pathol 40, 405–410 (1982). https://doi.org/10.1007/BF02932882
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02932882